The University of British Columbia

Brad Nelson

Anupam Singhal

Carl L G Hansen

Daniel Jay Da Costa

Carl Lars Genghis Hansen

Veronique Lecault

Marketa Ricicova

Kevin Albert Heyries

Hans Zahn

Oleh Petriv

Julie Nielsen

Kathleen Lisaingo

Carl L Hansen

Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.

Microfluidic devices and methods for use thereof in multicellular assays of secretion

Growing community of inventors

Victoria, Canada

Brad Nelson