Variagenics, Inc.

Avitech Diagnostics, Inc.

Borries W Kemper

Stefan Golz

Richard G Cotton

Rima Youil

Karin Birkenkamp-Demtroder

Rainer P Birkenbihl

Disclosed herein is a method for detecting a mismatch in a duplex nucleic acid, involving: a) contacting the duplex nucleic acid with a reactive agent under conditions which permit the agent to bind but not cleave a mismatch in said duplex nucleic acid; b) detecting binding of the agent to the duplex nucleic acid as an indication of the presence of a mismatch in the duplex nucleic acid; c) contacting the duplex nucleic acid with the reactive agent under conditions which permit the agent to cleave a mismatch in the duplex nucleic acid; and d) detecting a cleavage product as an indication of the presence of a mismatch in the duplex nucleic acid.

Mismatch detection techniques

Methods are disclosed for detecting one or more mutations in an isolated test nucleic acid by forming a heteroduplex with a homologous control DNA and contacting the heteroduplex with a resolvase capable of recognizing at least one single base pair mismatch within the heteroduplex. In preferred embodiments of the invention, the resolvase is bacteriophage T4 endonuclease VII.

Detection of mutation by resolvase cleavage

Disclosed are improved methods for the production and recovery of bacteriophage T4 endonclease VII.

Growing community of inventors

Koln, Germany

Borries W Kemper