The State University of New York

Boris Gorbovitski

Vera Gorfinkel

Dmytro Gudkov

Georgiy Gudkov

Dmitri N Gavrilov

Andriy Tsupryk

Evgeni A Kabotyanski

Marina Tcherevishnik

Ivan Tovkach

Dmitriy Gavrilov

A true random number generator including a light source configured to produce randomly distributed photons, a plurality of detection channels configured to receive the randomly distributed photons produced by the light source, each detection channel including a photon sensor configured to detect a receipt of at least one photon during successive integration time-periods and generate an output signal by assigning a value for each integration time-period based on whether at least one photon was received during each integration time-period, a signal conditioning unit configured to condition the output signal of each of the plurality of detection channels and generate a conditioned output signal for each of the plurality of detection channels, and a signal processing unit configured to combine the conditioned output signals and generate a true random number based on the combination of the conditioned output signals.

Quantum true random number generator

The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (PCR) on said volumes, detecting PCR amplification products, analyzing said detected PCR amplification products, performing absolute quantification of the PCR target and presenting said quantification results.

Absolute PCR quantification

This invention discloses a highly efficient method, system and apparatus for nucleic acid analysis, including sequencing (both automated re-sequencing and de-novo sequencing). The system is capable of sequencing DNA sizes ranging from fragments to mammalian size genomes having mouse draft quality at a much reduced cost. The system comprises a massive parallel capillary electrophoretic separation using two-dimensional monolith multi-capillary arrays (2D-MMCA). Sequence identification can be performed using fluorescent or otherwise labeled dideoxynucleotide-terminated DNA extension product generated by gel matrix-, or beads-, or substrate tethered-, or otherwise immobilized colonies of single template molecules. Cost reduction is a significant advantage over currently known methods because of: (i) using massively parallel sub-nanoliter volume reactions; and (ii) employing 2D-MMCAs that increase the throughput of the CE separation and detection by at least two orders of magnitude compared to the commercial high-throughput DNA machines.

Massively parallel 2-dimensional capillary electrophoresis

A fiberized single photon sensitive spectrometer based on a 32-channel PMT sensor is highly sensitive with broad detection dynamic range. The spectrometer enables accurate and high speed detection, identification and analysis of biological samples labeled with multiple fluorescent markers, such as compositions of multi-color fluorescence signals or radiation emitted by multiple fluorescence dyes. A fiberized optical input of the spectrometer allows an easy and efficient coupling to any measurement system based on fiber collection of the analyzed fluorescence. The spectrometer provides highly accurate DNA sequencing. A 32 channel PMT single photon detector has a detection dynamic range of more than 20 bits and has a frame rate of about 3300 frames per second. The dynamic range of the detector's pixels reaches 10photocounts per second.

Single photon spectrometer

The present invention is directed to a system and method for cross-talk cancellation for multi-lane fluorescence detectors. The invention may be implemented in accordance with a variety of systems, including systems for multi-capillary electrophoresis. The present invention is based on a special calibration procedure for determination of a channel cross-talk matrix and enables an accurate separation of the fluorescence emitted from individual capillary lanes. The proposed method for cross-talk calibration and removal is very useful for design and development of multi-lane single photon counting detection systems.

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Stony Brook, NY, United States of America

Boris Gorbovitski