Princeton University

University Technologies International, Inc.

Other

Quorex Pharmaceuticals, Inc.

Bonnie L Bassler

Michael G Surette

Lee R Swem

Martin F Semmelhack

Colleen T O'Loughlin

Scott Michael Ulrich

Stephan Schauder

Kevan M Shokat

Lark J Perez

Jeffrey D Stein

Justin E Silpe

Knut Drescher

Wai-Leung Ng

Shawn R Campagna

Michiko E Taga

Frederick M Hughson

Carol Dammel

Howard A Stone

Stephen R Cooper

Xin Chen

Ned Scott Wingreen

Douglas Tyler McKenzie

Douglas A Higgins

Megan Eileen Bolitho

Albert Siryaporn

Kristina M Kraml

Laura C Conrad-Miller

Michael J Federle

Yi Shen

Stephen T Miller

Karina B Xavier

Jianping Cong

Derrick H Lenz

Kenny C Mok

Described herein, are compositions and methods for preserving human milk, preventing rancidity of human milk, extending shelf life of human milk, and maintaining the nutritional properties of human milk. Specifically, the present disclosure provides, among other things, devices for storing preserved human milk and methods and compositions for preserving the same.

Methods, devices and compositions for preserving human milk

Recombinant phages that infect and kill bacterial hosts in response to user-defined inputs are provided. The components that encode the user-defined inputs can be combined, such that multiple inputs are maintained on a single recombinant phage, enabling precise control over the targeting strategy. The phages can be engineered to kill a specific bacterial species or multiple species simultaneously. Recombinant phages can also be engineered to harbor fluorescent and bioluminescent reporter genes that enable them to be used for tracking, detection, and in biosensing applications. Recombinant phages can also be used to lyse bacterial cells that produce recombinant proteins, as a rapid method to enable extraction and high-level purification of potentially valuable and/or industrially important proteins. Systems are provided that can be used to control the activity of a protein of interest, by taking advantage of an interaction between Qtip and a phage repressor protein.

System for protein inactivation and recombinant phages for targeted bacterial killing, infection, biodetection, and as a means of protein extraction

A structurally distinct and potent series of synthetic small molecule activators ofquorum sensing have been chemically synthesized. The small molecule activators reduce virulence in. Acyl pyrrole molecules displayed strong potency and stability, particularly 1-(1H-pyrrol-3-yl)decan-1-one.

Heterocycle analogs of CAI-1 as agonists of quorum sensing in vibrio

Antivirulence strategies to combat, are described. One strategy encompasses synthesis of a series of compounds that inhibit the production of pyocyanin, a redox-active virulence factor produced by this pathogen. A related strategy encompasses synthesis of compounds that inhibit the twoquorum-sensing receptors, LasR and RhlR, inhibit production of pyocyanin, and inhibit biofilm formation.

Molecules and compositions that inhibit gram negative bacteria and their uses

Methods and systems of measuring biofilms and/or biofilm streamers are presented. The system has at least one channel, a biofilm streamer promotion element, a fluid capable of moving along the channel, wherein the fluid has a flow driven by a controlled pressure; and a measuring element capable of measuring a flow rate of the fluid through the channel. Additionally, methods of screening for compound(s) that promote and/or inhibit biofilm and/or biofilm streamers using this system are also presented. Uses of the system, methods and identified compounds are presented for medical and/or industrial environments.

Systems and methods to detect biofilm streamer growth and their uses

Using a whole-cell high-throughput screen, eleven molecules were identified that activatequorum sensing (QS). Eight molecules are receptor agonists and three molecules are antagonists of LuxO, the central NtrC-type response regulator that controls the globalQS cascade. Pro-QS molecules are used for the development of novel anti-infectives.

Broad spectrum pro-quorum-sensing molecules as inhibitors of virulence in vibrios

A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryotefrom quorum-sensing-mediated killing by the bacterial pathogen. The saving ofdemonstrates the use of these molecules as small molecule antimicrobials.

Small molecule antagonists of bacterial quorum-sensing receptors

Methods are provided for identifying molecules that can be used to positively and negatively manipulate quorum-sensing-mediated communication to control bacterial behavior. Small-molecule antagonists that disrupt quorum-sensing-mediated activities are identified. Methods are provided for disrupting detection of acyl-homoserine lactone autoinducer in Gram-negative bacteria by contacting the bacteria with the antagonists. Methods of inhibiting quorum sensing-mediated activity in Gram-negative bacteria are provided wherein the activity is pathogenicity, bioluminescence, siderophore production, type III secretion, or metalloprotease production.

Inhibition of quorum sensing-mediated processes in bacteria

A bacterial autoinducer, CAI-1, was purified and its structure identified. Methods for synthesis of the autoinducer and its analogues were elucidated. Methods of using the autoinducer or its analogues for treating bacterial pathogenicity and bio film formation are described. Methods for prevention and treatment of cholera are described. Synthetic (S)-3-hydroxytridecan-4-one functions as well as natural CAI-1 in repressing production of the virulence factor toxin co-regulated pilus (TCP). Strategies are described to manipulate bacterial quorum sensing in the clinical arena.

Identification of bacterial autoinducer and use in treating bacterial pathogenicity

Growing community of inventors

Princeton, NJ, United States of America

Bonnie L Bassler