Korea University Industry and Academy Cooperation Foundation

Korea University Research and Business Foundation

Korea University Industrial Academic Collaboration Foundation

Sfc Co., Ltd.

Korea University Foundation

Bong-Rae Cho

Eun Sun Kim

Hoon Jai Chun

Chang Su Lim

Ji Yeon Kim

Sun Young Yang

Hwan Myung Kim

Dong Eun Kang

Seung-Joon Jeon

Sajal Kumar Das

Min-Haeng Cho

The present disclosure relates to a two-photon probe for in-vivo imaging, having high selectivity for copper(II) ion, and a method for quantitatively estimating copper(II) on (Cu) in vivo using the same. The two-photon probe according to the present disclosure can detect intracellular free copper(II) on for a long period of time with a penetration depth greater than 90 &#x3bc;m in living cells and tissues without the problems of mistargeting and photobleaching and can quantitatively estimate copper(II) on concentration in vivo using the ratio of blue emission intensity at 400-450 nm and red emission intensity at 550-650 nm.

Two-photon probe for detecting copper(II) ion and quantitative estimation of copper(II) ion in human tissue using the same

The present disclosure relates to a two-photon fluorescent dye for imaging total sulfide in vivo and a method for quantitatively estimating total sulfide concentration in vivo using the same. The two-photon fluorescent dye, which includes a compound of [Chemical Formula 1], can detect hydrosulfide ion and image total sulfide and allows quantitative estimation of total sulfide concentration in vivo.

Two-photon fluorescent probes for imaging of total sulfide in live cell and tissue, and quantitative estimation of total sulfide concentration using the same

A two-photon probe for real-time monitoring of intracellular free zinc ions is provided. The two-photon probe is represented by Formula 1: wherein R is H or OCH. The two-photon probe has high selectivity for Znand enables very effective and long-term monitoring of intracellular free Znpresent in the deep tissue. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular free zinc ions using the two-photon probe.

Two-photon probe for real-time monitoring of intracellular free zinc ions, method for preparing the probe and method for real-time monitoring of intracellular free zinc ions using the probe

A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20&#x2dc;50-fold TPEF enhancement in response to Ca, has a dissociation constant (K) of 0.14&#xb1;0.02 to 0.25&#xb1;0.03 &#x3bc;M, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Caprobes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Cain live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 &#x3bc;m in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe.

Two-photon probe for real-time monitoring of intracellular calcium ions, method for preparing the probe and method for real-time monitoring of intracellular calcium ions using the probe

Provided are two-photon fluorescent probes for imaging acidic vesicles in live cells and tissue. The probes are represented by The probes can selectively bind to vesicles in cytosol to emit two-photon excited fluorescence with high intensity. Therefore, the use of the probes enables effective imaging of acidic vesicles. Further provided is a method for imaging acidic vesicles in live cells and tissue using the probes.

Two-photon fluorescent probes for acidic vesicles in live cells and tissue and method of imaging acidic vesicles in live cells and tissue using the same

A two-photon probe for real-time monitoring of intracellular magnesium ions is provided. Specifically, the two-photon probe is represented by Formula 1:  The two-photon probe is very suitable for real-time imaging of intracellular magnesium ions. The two-photon probe shows 17-fold two-photon excited fluorescence enhancement in response to Mg, which is 7-fold stronger than commercial probes, thus enabling staining of cells in a greatly reduced amount. In addition, the two-photon probe has a sufficiently low molecular weight to stain cells and is very suitable for monitoring Mgions present in the deep tissue. Furthermore, the two-photon probe can be effectively used for the quantitative as well as qualitative detection of intracellular magnesium ions. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular magnesium ions using the two-photon probe.

Two-photon probe for real-time monitoring of intracellular magnesium ions, method for preparing the two-photon probe and method for real-time monitoring of intracellular magnesium ions using the two-photon probe

Two-photon dyes for real-time imaging of lipid rafts are provided. The two-photon dyes emit fluorescence with high intensity in the environments of cell membranes and can be used to clearly discriminate the hydrophobic and hydrophilic domains of the cell membranes. Therefore, the use of the two-photon dyes enables satisfactory imaging of actual lipid raft domains. In addition, since the two-photon dyes show no cytotoxicity, they can be suitably used in bioimaging applications, for example, real-time imaging of lipid rafts over a long period of time.

Two-photon dyes for real-time imaging of lipid rafts

The present invention relates to 1,3,5-tricyano-2,4,6-tris(vinyl)benzene derivatives and method for preparing the same. The 1,3,5-tricyano-2,4,6-tris(vinyl)benzene derivatives can be prepared by refluxing 1,3,5-tricyanomesitylene with N-formylamine dimethylacetal or substituted benzaldehyde, or by the Wittig reaction of 1,3,5-tricyano-2,4,6-tris[(diethoxyphosphoryl)methyl]benzene with substituted benzaldehyde. The 1,3,5-tricyano-2,4,6-tris(vinyl)benzene derivatives exhibit large first hyperpolarizability in solution and significant second harmonic generation (SHG) in the powder state, and are useful as optical devices such as electro-optic modulators, optical switch, or the like for treating optical signal in optical communication industry.

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Seoul, South Korea

Bong-Rae Cho