Korea Research Institute of Bioscience and Biotechnology

Korea Institute of Science and Technology

Dongkook Pharmaceutical Co., Ltd.

Bionano Health Guard Research Center

Haesung Bio Co., Ltd.

Korea Advanced Institute of Science and Technology

Somar Corporation

Micobiomed. Co., Ltd.

Nanobiotech Co., Ltd.

Bong Hyun Chung

Yong Taik Lim

Jung Hyun Han

Sehoon Kim

Young Hun Seo

Sang Ki Rhee

Youngsun Kim

Jeong Min Lee

Myung Kyu Lee

Sang Jeon Chung

Seong U Kim

Kyung Mi Park

Jong Min Choi

Kyoung Sook Park

Hyo Jin Kang

So Yeon Yi

Yongwon Jung

Ui Jin Lee

Heejun Lee

Ho Nam Chang

Toshihiro Akaike

Eun Gyo Lee

Jin Young Jeong

Jeong Hee Moon

Yoonkyung Kim

Ho Jae Lee

Myung K Kim

Nam Woong Song

Byung Kwon Kim

Jung Hoon Sohn

Hye-Youn Jung

Young Hoon Park

Hirohiko Ise

Yeon Woo Ryu

Eui Sung Choi

Bang Sil Choi

Kyung Jin Lee

Ga Bi Lee

Jinyoung Jeong

Young-mi Lee

Sun-Jung Kim

Yu-Jin Jeong

Eun Kyung Lim

Sang Kyu Kim

Hye Jung Park

Sun-Ok Jung

Jung Sun Kwon

Han Seung Lee

Young-Mi Lee

Deok Joong Youn

Abbas Mokhtarieh Amir

Hyun Ah Kana

Hae Don Lee

Sun Ah Yang

Su Min Ko

Byung Moon Kim

Soo Wan Nam

Nyeon Sik Eum

Moon Sun Hahm

So Yoen Yi

A method for detecting biomaterial by means of a dye having a linear upconversion fluorescent property is provided. The method includes the steps of: i) preparing a fluorophore having a linear upconversion fluorescent property; ii) reacting the fluorophore and biomaterial to obtain a reaction complex thereof; iii) exciting the reaction complex by means of a light source having a longer wavelength than the maximum light-emitting wavelength of the fluorophore; and iv) detecting and measuring the light-emitting signal having a shorter wavelength than the wavelength of the excited light emitted from the excited reaction complex. A system and a kit for detecting biomaterial using a dye having a linear upconversion fluorescent property are also provided.

Method for detecting biomaterial using linear upconversion fluorescent property

The present invention relates to a composition for detecting microbial contamination comprising a preparation for detecting nucleases, and a use thereof. A probe for measuring nucleic acid double-stranded nucleases, according to the present invention, may detect the comprehensive nucleic acid degradation capability of nucleases, whereby it is possible to quickly and precisely detect and quantify live microorganisms in a sample in a simple manner. Moreover, since the probe of the present invention is characterized in that signals of fluorescence consistently increase; is capable of measuring live microorganisms; and consists of a double-stranded nucleic acid, the probe is excellent for storage in a kit or a cartridge. As such, it is expected that the probe of the present invention may be used to easily and simply measure and compare the degree of contamination of microorganisms in an environment.

Composition for detecting microbial contamination comprising preparation for detecting nucleases, and use thereof

The present invention relates to a probe for detecting bacteria using a peptidoglycan-binding protein, and a method for preparing the same. Also, the present invention relates to a method for detecting bacteria using the probe. The probe for detecting bacteria according to the present invention can specifically detect bacteria. That is, the probe according to the present invention can clearly distinguish between yeast and bacteria and can detect both Gram-negative and Gram-positive bacteria, and thus is expected to be usable in various fields as a universal probe for detecting bacteria. Further, use of the probe allows bacteria to be detected by identifying only fluorescence development without an additional enzymatic treatment, thereby enabling a simple and quick bacterial detection. In particular, the probe is expected to be effectively usable in the food industry where quick bacterial detection is required.

Probe for detecting bacteria using peptidoglycan-binding protein, and use thereof

Provided is a methionyl tRNA synthase (MRS) for the biosynthesis of a photomethionine-labeled protein and a method for preparing a photoactivatable protein G variant using same and, more particularly, to an MRS variant in which alanine at the position of 12is substituted with glycine, leucine at the position of 13by serine, tyrosine at the position of 260by phenylalanine, isoleucine at the position of 297by valine, and histidine at the position of 301by leucine from the N-terminal of the amino acid sequence of a wild-typemethionyl tRNA synthase. The MRS variant effectively confirms the biosynthesis of a photomethionine (pM)-labeled target protein and thus can be utilized for the biosynthesis of a pM-labeled target protein. In addition, a pM-introduced protein G variant, in which a plasmid encoding the MRS variant (MRS5m) and a PG-C3 plasmid, in which, in the third immunoglobulin G binding region of protein G, positions of 32, 35, and 40are substituted with a methionine (Met) residue and a position of 37by an arginine (Arg) residue, are introduced intoand then refined, has a specific covalent bond with an antibody when subject to UV irradiation, and thus the pM-introduced protein G variant using the MRS variant can be utilized for producing a highly sensitive biochip, biosensor, or cell-capturing chip.

Methionyl tRNA synthetase for biosynthesis of photomethionine-labeled protein and method for preparing photoactive protein G variant using same

A sugar chain-containing polymer that enables targeting to the lesion area of liver fibrosis and is useful for imaging, diagnosis and therapy of liver fibrosis; and a sugar chain-containing polymer complex comprising the polymer as a carrier for an anionic substance useful fix therapy and the like; are provided. The polymer is a sugar chain-containing polymer which is a cationic polymer comprising an amine, which polymer comprises N-acetylglucosamine bound thereto. The polymer preferably has a disulfide bond. The polymer preferably has a structure in which polyethyleneimine is linked via a disulfide bond.

Sugar chain-containing polymer, and sugar chain-containing polymer complex

The present invention relates to a novel compound isolated fromsp., and more particularly to a novel compound isolated fromsp. and a composition for preventing or treating diabetes and its complications comprising the compound as an active ingredient. The novel compound isolated fromsp. according to the present invention has excellent effects on lowering blood sugar, promoting insulin secretion, inhibiting VEGF expression, and so on. Thus, the present invention not only functions to prevent or treat diabetes and its complications, but also functions to promote treatment effects when treated together with conventional diabetes medicines.

Compound isolated from quamoclit, and composition for preventing or treating diabetes containing the compound as an active ingredient

The present invention relates to asymmetric liposomes for high encapsulation efficiency of nucleic acids and hydrophilic anionic compounds, and to a method for preparing same, and specifically, to asymmetric liposomes consisting of a cationic lipid having a small head group as an internal lipid and a neutral or PEGylated lipid having a big head group as an external lipid, wherein nucleic acids and/or anionic compounds are encapsulated in the internal lipid. According to the present invention, asymmetric liposomes, in which nucleic acids and hydrophilic anionic compounds are encapsulated with high efficiency, may be prepared, and thus the same may be used for various purposes, such as gene therapy, and the delivery of hydrophilic anionic drugs which are difficult to prepare as prodrugs, and drug delivery, imaging, etc. can be carried out by encapsulating a fluorescent contrast agent in the liposome.

Asymmetric liposomes for the highly efficient encapsulation of nucleic acids and hydrophilic anionic compounds, and method for preparing same

A simple and easy method is described for preparing a water-soluble fluorescent fullerene derivative with strong fluorescence and hydrophilicity, by mixing fullerene and a ligand containing a terminal hydroxyl group in a first solvent and reacting the mixture in the presence of a catalyst. Such preparation method enables the intensity and wavelength of fluorescence to be easily controlled depending on the amount of fullerene and the type of catalyst that are utilized. The prepared fluorescent fullerene derivative contains a biocompatible ligand, and thus is useful as a biological fluorescent dye. As a result of its fluorescence and excellent solubility in solvent, the fluorescent fullerene derivative is useful in biological, medical, nanotechnology, and other fields.

Water-soluble fluorescent fullerene derivative, and preparation method thereof

Provided is a microneedle unit comprising a case having a space accommodating a fluid and a fluid channel through which the fluid is discharged, a microneedle coupled to a lower portion of the case, a base cover disposed at the lower portion of the case, having a hole through which the microneedle passes, and being vertically movable, a guide pin coupled to the base cover and configured to open and close the fluid channel, and an elastic member configured to impart a restoring force such that the base cover is moved upward by an external pressure and then returns downward again. Therefore, the microneedle unit, which is provided to deliver a fluid into the skin with no pain and no scar, can control injection of the fluid using the guide pin, and repeatedly and conveniently control injection of the fluid using the elastic member.

Microneedle unit capable of controlling fluid delivery

Provided are a coenzyme Q10 nanoparticle, a method of preparing the same and a composition having the nanoparticle. According to the present invention, Coenzyme Q10 may be dissolved in only a water-miscible organic solvent, and easily made into a nano-sized particle and solubilized under a low energy condition, for example, by simple stirring. The coenzyme Q10 may be dispersion-stabilized by an amino acid or protein. The coenzyme Q10 is formed in a nano-sized particle and solubilized, an absorption rate may be increased and simultaneously deliver the amino acid and protein with the nanoparticle. Thus, the coenzyme Q10 nanoparticle can be effectively used in food, cosmetics and medicine.

Coenzyme Q10 nanoparticles, preparation method thereof and composition containing said nanoparticles

The present invention relates to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, and more particularly, to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, including a first step of dissolving a biodegradable polymer in a non-volatile polar organic solvent, a second step of adding poorly soluble drugs to water and the biodegradable polymer solution to produce a dispersion, and a third step of adding the dispersion to emulsifier solutions in a batch under the condition of low mechanical energy level stirring. The polymeric nanoparticles of the present invention is capable of manufacturing nano-sized small and uniform polymeric nanoparticles through a simple method of employing a non-volatile polar solvent, especially a solvent having a polarity similar to that of water, as a solvent for a mixture solution of biodegradable polymer and poorly soluble materials, and using a low mechanical energy condition and batch-adding of dispersion in an emulsifying process. The polymeric nanoparticles of the present invention are advantageous in that the dissolution rate of the poorly soluble drugs contained in polymeric particles is dramatically improved, and the poorly soluble drugs are gradually and steadily released and maintained at a constant density over a long period of time.

Method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs

The present invention relates to a multimodal imaging method using a nano-emulsion comprising optical nano-particles and perfluorocarbons, and more particularly, to a method of obtaining multimodal images using a nano-emulsion consisted of perfluorocarbons for multispectral magnetic resonance imaging (MRI) and nano-particles for multicolor fluorescent detection. In the multimodal imaging method, various multispectral MRI images and multicolor fluorescent rays can be obtained by varying the kind and combination of perfluorocarbons and the kind and combination of optical nano-particles, so that multiplexed analysis is possible. Accordingly, the multimodal imaging method can be applied to various fields, including biological and medical fields in which studies on cell molecular imaging has been conducted.

Multimodal imaging method using nano-emulsion comprising optical nano-particles and perfluorocarbons

The present invention relates to a perfluorocarbon nanoemulsion containing quantum dot nanoparticles and a method for preparing the same, more specifically, relates to a method for preparing a multifunctional perfluorocarbon nanoemulsion which simultaneously shows the fluorine-based magnetic resonance properties of perfluorocarbon and the optical properties of quantum dot nanoparticles, the method comprising dispersing quantum dot nanoparticles, modified with a fluorine end group, in perfluorocarbon, and then adding an aqueous emulsifier solution containing lipid to the dispersion. The disclosed perfluorocarbon nanoemulsion containing quantum dot nanoparticles has excellent biocompatibility and simultaneously shows optical properties and magnetic resonance properties, and thus can be used as a contrast agent for multi-mode molecular imaging. Accordingly, the nanoemulsion can be used to study cell and small-animal molecular imaging in various fields, including biological and medical fields.

Perfluorocarbon nano-emulsion containing quantum dot nanoparticles and method for preparing the same

The present invention relates to fullerene-silica nanoparticles with improved fluorescence, a preparation method of the fullerene-silica nanoparticles, and use thereof. More specifically, the present invention relates to fullerene-silica nanoparticles with improved fluorescence in which fullerene and silica are covalently linked, a preparation method of the fullerene-silica nanoparticles, and use thereof. The preparation method of the fullerene-silica nanoparticles comprises the steps of: adding a surfactant to a non-polar organic solvent and a polar solvent and stirring them to form reverse micelles (step 1); adding fullerene to the reverse micelles formed in the step 1 and stirring them (step 2); and adding a silica precursor and a catalyst to a reaction solution containing the fullerene prepared in the step 2 and stirring them to prepare fullerene-silica nanoparticles (step 3). According to the present invention, fullerene-silica nanoparticles in the form of a uniform spherical shape of tens of nanometers can be prepared. As such prepared fullerene-silica nanoparticles have a large surface area due to a nanometer-sized structure, they have good reactivity. Compared with heavy metals or metal nanoparticles, the fullerene-silica nanoparticles, because of using silica, are harmless to a living body. Further, as the fullerene-silica nanoparticles exhibit strong fluorescence, they can be used as a contrast agent for in-vivo imaging or as a drug delivery carrier.

Fullerene-silica nanoparticles with improved fluorescence, preparation method thereof and use thereof

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Daejeon, South Korea

Bong Hyun Chung