China National Seed Group Corporation, Ltd.

Other

Bolin Liu

Chao Tan

Wanggen Zhang

Chonglie Ma

Jieting Xu

Yu Han

Peter Ordentlich

Liwen Xiong

Qianqian Yang

Qiuming She

Qin Wen

Samuel S M Sun

Xuxia Wang

Peixiuzi Tian

Dongming Nie

Long Qiu

Yuxiang Jing

Bob Goodenow

Xiaoping Huang

An insect-resistant herbicide-tolerant corn transformation event, and a related creation method, a detection method, and application thereof are provided herein. Using the corn inbred line Xiang 249 as a receptor, by means of agrobacterium-mediated genetic transformation, obtaining a corn plant with an exogenous gene insert inserted at a specific genomic locus, the exogenous gene insert comprising the following three genes: an insect-resistant gene, a glufosinate resistant gene, and a glyphosate resistant gene. In the obtained transformation event, the inserted exogenous genes are positioned at a non-functional locus of the corn genome, and do not affect the expression of the other genes of the receptor plant, such that the transgenic corn plant maintains good agronomic traits whilst acquiring insect resistance and herbicide tolerance.

Insect-resistant herbicide-tolerant corn transformation event

The present disclosure relates to a gene sequence Cry1Ab/Cry1AcZM, an expression cassette containing the gene, an expression vector, a host cell and the use thereof in plant breeding. The protein encoded by an insect-resistant gene according to the disclosure can be expressed in a monocotyledonous plant and further used for culturing an insect-resistant transgenic monocotyledonous plant harboring transgenes Cry1Ab/Cry1AcZM. Bioassay tests prove that the modified and synthesized gene sequence Cry1Ab/Cry1AcZM and the expression product of the constructed vector according to the disclosure have a killing effect on lepidopteran pests.

Gene for encodingcrystal proteins, and use thereof

A lysine-rich protein gene has been cloned from winged bean,,, and its DNA sequence and deduced protein sequence are provided. Methods for transforming plant cells are provided that mature into plants having elevated levels of lysine in their seeds.

Lysine rich protein from winged bean

Growing community of inventors

Beijing, China

Bolin Liu