Life Technologies Corporation

Other

Boli Huang

Kelly Li

Ioanna Pagani

Steven Menchen

Barnett B Rosenblum

Zhaochun Ma

Pius Brzoska

Paul M Kenney

Jer-Kang Chen

Nitin Puri

Joe Y Lam

Kamini Varma

Shoeb Khan

Evan Diamond

Sunali Patel

Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.

Compositions, methods and kits for microorganism detection

Nucleic acid reagents and corresponding methods of using the same for monitoring and evaluating nucleic acid extraction and amplification reactions.

Nucleic acid extraction and amplification controls and methods of use thereof

Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3' hydroxyl group. Methods of sequencing DNA using the substrates are also described.

Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use

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Palo Alto, CA, United States of America

Boli Huang