University of Pennsylvania

University of Minnesota

University of Illinois

Bohdana Marie Discher

Frank Steven Bates

Brett R Goldsmith

Alan T Johnson

Dennis E Discher

You-Yeon Won

Daniel A Hammer

James C-m Lee

Joseph J Mitala

Brian Yichiun Chow

Stephen G Sligar

Timothy H Bayburt

Nathan M Ennist

Goutham Kodali

Peter Leslie Dutton

Molly Marie Sheehan

Christopher Moser

Joshua Mancini

Michael Magaraci

Ye Lu

De novo artificial protein based reporters that may be expressed in eukaryotic (e.g., mammalian) cells and methods of using the same are provided herein.

Artificial proteins and compositions and methods thereof

Provided are devices and methods featuring a nanoelectronic interface between graphene devices (for example, field effect transistors or FETs) and biomolecules such as proteins, which in turn provides a pathway for production of bioelectronic devices that combine functionalities of the biomolecular and inorganic components. In one exemplary application, one may functionalize graphene FETs with fluorescent proteins to yield hybrids that respond to light at wavelengths defined by the optical absorption spectrum of the protein. The devices may also include graphene in electronic communication with a biomolecule that preferentially binds to a particular analyte.

Graphene-biomolecule bioelectronic devices

The present invention provides biomimetic sensor devices that utilize proteins&#x2014;such G-protein coupled receptors&#x2014;and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.

Biomimetic chemical sensors using nanoelectronic readout of olfactory receptors

The present invention provides biomimetic sensor devices that utilize proteins&#x2014;such as G-protein coupled receptors&#x2014;and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.

The present invention provides biocompatible vesicles comprising semi-permeable, thin-walled encapsulating membranes which are formed in an aqueous solution, and which comprise one or more synthetic super-amphiphilic molecules. When at least one super-amphiphile molecule is a block copolymer, the resulting synthetic vesicle is termed a 'polymersome.' The synthetic, reactive nature of the amphiphilic composition enables extensive, covalent cross-linking of the membrane, while maintaining semi-permeability. Cross-linking of the polymer building-block components provides mechanical control and long-term stability to the vesicle, thereby also providing a means of controlling the encapsulation or release of materials from the vesicle by modifying the composition of the membrane. Thus, the encapsulating membranes of the present invention are particularly suited for the reliable, durable and controlled transport, delivery and storage of materials.

Cross-linked polymersomes and related encapsulating membranes

Polymersomes and related encapsulating membranes

The present invention provides biocompatible vesicles comprising semi-permeable, thin-walled encapsulating membranes which are formed in an aqueous solution, and which comprise one or more synthetic super-amphiphilic molecules. When at least one super-amphiphile molecule is a block copolymer, the resulting synthetic vesicle is termed a &ldquo;polymersome.&rdquo; The synthetic, reactive nature of the amphiphilic composition enables extensive, covalent cross-linking of the membrane, while maintaining semi-permeability. Cross-linking of the polymer building-block components provides mechanical control and long-term stability to the vesicle, thereby also providing a means of controlling the encapsulation or release of materials from the vesicle by modifying the composition of the membrane. Thus, the encapsulating membranes of the present invention are particularly suited for the reliable, durable and controlled transport, delivery and storage of materials.

Growing community of inventors

Philadelphia, PA, United States of America

Bohdana Marie Discher