Synthetic Genomics, Inc.

Codex Dna, Inc.

Bogumil J Karas

Hamilton O Smith

J Craig Venter

Daniel Glenn Gibson

Clyde A Hutchison

Ray-Yuan Chuang

Vladimir N Noskov

Krishna Kannan

Lin Ding

Kim S Wise

John I Glass

Chuck Merryman

Christopher L Dupont

Philip D Weyman

Andrew E Allen

Hutchison Clyde A, Iii

Yo Suzuki

Methods for generating synthetic genomes, for example synthetic genomes having desired properties or viable genomes of reduced size, are disclosed. Also disclosed are synthetic genomes produced by the methods disclosed herein and synthetic cells containing the synthetic genomes disclosed herein.

Generation of synthetic genomes

The present invention relates to the seminal discovery of a highly efficient method of transforming algal cells. Specifically, the invention relates to a novel method of delivering a plasmid containing a nucleic acid molecule to algal cells by bacterial conjugation wherein the plasmid remains episomal in the algal cell through multiple generations.

High efficiency method for algal transformation

The presently disclosed invention relates to methods of transferring large nucleic acid molecules or a genome from one cell (the donor) into heterologous host cells in the presence of a crowding agent. The method allows for greater ease and efficiency of transfer of genetic material. Introduction of the donor genetic material into the recipient host cells also allows for manipulation of the donor nucleic acid molecule or genome within the host cells. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

Crowding agent-induced nucleic acid transfer into a recipient host cell

Growing community of inventors

London, Canada

Bogumil J Karas