Tianjin University of Science and Technology

Henan Julong Biological Engineering Co., Ltd

Bochao Liu

Ning Chen

Qingyang Xu

Xiaoguang Fan

Yuhang Zhou

Jing Li

Xiaodong Liu

Shuai Liu

Chaochao Sun

Yongchao Liu

Jiajia Teng

Mengtao Zhang

Yuanqing Ji

The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using angenetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on anW3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

Method for producing L-theanine via fermentation by a genetically engineered bacterium and the application thereof

Genetically engineered bacterium ofand method for fermentation production of L-theanine thereof

Growing community of inventors

Henan, China

Bochao Liu