National Institute of Fisheries Science

Republic of Korea (National Fisheries Research and Development Institute)

National Fishery Products Quality Management Service

Bo-Young Jee

Seong Don Hwang

Myoung Ae Park

Miyoung Cho

Jee Youn Hwang

Jung Soo Seo

Jae Hun Cheong

Sang Jung Ahn

Mun Gyeong Kwon

Hyun Kook Cho

Maeng Hyun Son

Mi So Seong

Kwang Il Kim

Mi Young Cho

The present invention relates to methods of identifying viral hemorrhagic septicemia virus (VHSV) and detecting mutations in VHSV by use of a PNA probe that binds specifically to VHSV non-virion gene and by analysis of the melting pattern of the PNA probe. According to the present invention, a mutation in a virus that causes infectious disease in aquatic organisms can be detected in a simple, rapid and accurate manner, infection of fish with the virus can be detected, and a mutation in the NV gene can be detected.

Method for detecting mutation in viral hemorrhagic septicemia virus (VHSV) non-viron (NV) gene

Genetic markers are described for discriminating or detecting viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same is disclosed, in which the method includes selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish are infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.

Genetic markers for discrimination and detection of a virus causing red sea bream iridovirus disease which is an infectious aquatic organism disease, and method of discriminating and detecting the virus using the same

Genetic markers for discrimination and detection of gray SPH region on Koi herprsvirus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same

Genetic markers for discrimination and detection of red sea bream iridovirus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same

A biomarker composition includes, as an active ingredient, one or at least two polypeptides having 5 to 120 consecutive amino acids that are selected from the group consisting of an amino acid in which serine (S) at the 56position is substituted with leucine (L), an amino acid in which serine (S) at the 8position is substituted with asparagines (N), an amino acid in which threonine (T) at the 81position is substituted with alanine (A), an amino acid in which valine (V) at the 88position is substituted with alanine (A), an amino acid in which glycine (G) at the 117position is substituted with asparatic acid (D), and an amino acid in which glutamic acid (E) at the 119position is substituted with lysine (K), within an amino acid sequence of VHSV represented by SEQ ID NO: 1.

Biomarker for detecting highly pathogenic viral hemorrhagic septicemia virus and diagnostic method

The present invention relates to a single-nucleotide polymorphism (SNP) for determining genotypes specific to the regions from which VHSV has been isolated, to PNA for identifying same and a method for identifying, by using same, the single-nucleotide polymorphism (SNP) for determining genotypes specific to the regions from which VHSV has been isolated and, more specifically, to PNA and a kit capable of detecting the single-nucleotide polymorphism mutations of C755A and A756G of the VHSV G-protein by using PNA comprising a sequence of SEQ ID NO: 1. The present invention enables an easy, rapid and accurate identification of genotypes specific to the regions from which VHSV has been isolated, by using PNA having an excellent binding affinity to DNA so as to make each genotype exhibit a different melting temperature.

Probes for identifying geographical distribution and molecular epidemiology of viral hemorrhagic septicemia virus (VHSV) and uses thereof

The present invention relates to genetic markers for discrimination and detection of viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same, and more particularly to a method for discriminating or detecting viruses causing infectious aquatic organism diseases, the method comprising: selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish would be infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.

Genetic markers for discrimination and detection of bercoiver tk region on koi herpesvirus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same

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Busan, South Korea

Bo-Young Jee