Arizona State University

Bo Wang

Deirdre Meldrum

Weiwen Zhang

David Nielsen

Methods of pre-methylation of foreign DNA to improve genetic transformation in cyanobacterium. Two Type II methyltransferase-encoding genes, i.e., M (sll0729) and C (slr0214), were cloned from the chromosome ofsp. PCC 6803 (hereafter6803) and expressed inthat harbors the integrative plasmid pBS-SPtK or pJU105. After pre-methylation in, the integrative plasmids were extracted and used for transformation of6803. The results showed that expression of slr0214 in the integrative-plasmid-harboringcells before DNA preparation resulted in orders of magnitude higher efficiency in the following integrative transformation of6803.

Premethylation of DNA for high efficiency transformation of cyanobacteria

Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacteriumsp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB fromwas the most effective, producing up to 533.4&#xb1;5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and COas sole energy and carbon sources by engineered cyanobacteria is demonstrated.

Photosynthetic production of 3-hydroxybutyrate from carbon dioxide

Construction and expression of synthetic pathways to produce (5) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacteriumsp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB fromwas the most effective, producing up to 533.4&#xb1;5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and COas sole energy and carbon sources by engineered cyanobacteria is demonstrated.

Growing community of inventors

Tempe, AZ, United States of America

Bo Wang