Chinese Academy of Agricultural Sciences

Bo Jiao

Yu Zhang

Qiang Wang

Li Liu

Hui Hu

Aimin Shi

Hongzhi Liu

Xiaoyong Xia

Qin Guo

Jinchuang Zhang

Shi Meng

Weiming Fu

Yanzheng Ge

The present invention relates to a margarine substitute loaded with trans-resveratrol/glycoside and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a peanut protein dispersion, adding a transglutaminase thereto, performing a cross-linking reaction under a water bath condition, and heating up to obtain a monolithic gel; continuing to add water to the monolithic gel, shearing the resultant at a high speed, homogenizing at a high pressure, performing ultrasonication, and adjusting pH to obtain a microgel particle dispersion; (2) adding a trans-resveratrol/glycoside to an edible oil in the dark, stirring well, continuing to add the microgel particle dispersion obtained in step (1), and shearing the resultant at a high speed to obtain a high internal-phase Pickering emulsion, i.e. a margarine substitute loaded with trans-resveratrol/glycoside. The trans-resveratrol/glycoside in the margarine substitute loaded with trans-resveratrol/glycoside of the present invention maintains relatively high activity and can solve a series of industrial problems brought by trans fat.

Margarine substitute loaded with trans-resveratrol/glycoside and preparation method thereof

A method for preparing a Pickering emulsion using a peanut protein isolate includes preparing a peanut protein isolate dispersion liquid from a peanut protein isolate solution as a raw material; preparing a mixed dispersion of protein and polysaccharide using a polysaccharide solution and the peanut protein isolate dispersion; adding transglutaminase to the mixed dispersion of protein and polysaccharide, preparing a monolithic gel by cross-linking reaction; preparing a microgel particle dispersion by using the monolithic gel as a raw material; and further adding the microgel particle dispersion to an edible oil to obtain a Pickering emulsion. During the preparation process, no inorganic material is added, and the obtained Pickering emulsion has good biosafety and strong biocompatibility. The prepared Pickering emulsion can keep stable at room temperature for 30 days or more, and can be used as a delivery system for fat-soluble and photosensitive active substances.

Pickering emulsion prepared using peanut protein isolate and preparation method thereof

The present application relates to a method for simultaneously detecting four isomers of resveratrol in peanut by using ultra performance liquid chromatography during the operation procedure and accurately controlling the detection conditions. The method of the invention can achieve continuous sample injection, and perform sample analysis in batch. The detection time for each sample is only about 10 min, which greatly improves the detection efficiency. Further, the ultra performance liquid chromatography has high sensitivity and a low detection limit. The method of the invention adopts ethanol extraction under heating in the earlier stage of extraction, and controls the temperature of the extraction, so as to achieve effective extraction of four isomers of resveratrol, time saving, and efficient extraction and separation.

Method for simultaneously detecting four isomers of resveratrol in peanut

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Beijing, China

Bo Jiao