Recursion Pharmaceuticals, Inc.

Blake C Borgeson

Mason L Victors

Ronald Wakim Alfa

David Healey

Cedric St-Jean-Leblanc

Chris Gibson

Ian Quigley

Berton Allen Earnshaw

Peter Foster McLean

Renat Nailevich Khaliullin

Nathan Henry Lazar

Katie-Rose Skelly

Systems and methods for determining whether a set of test perturbations discriminates over a null distribution for an on target effect against a first component of an entity are disclosed. The perturbations are perturbations of the first component and the entity comprises a plurality of components. For each perturbation in the set, a corresponding vector comprising a plurality of elements, is obtained. Each element comprises a distribution metric of measurements of a feature across instances of the entity upon exposure to the respective perturbation or (ii) a distribution metric of a respective dimension reduction component computed using the measurement of the plurality of features across instances of the entity upon the perturbation exposure. A composite metric is computed, using the vectors, and compared to a null distribution. When the composite metric is differentiated from the null distribution, the set of perturbations is deemed to discriminate the on target effect against the first component over the null distribution.

Systems and methods for discriminating effects on targets

Methods and systems for evaluating a query perturbation, in a cell based assay representing a test state, are provided. Control data points having dimensions representing measurements of different features across control cell aliquots are obtained. Test data points having dimensions representing measurements of different features across test cell aliquots are obtained. A composite test vector is computed between measures of central tendency across the control data points and measures of central tendency across the test data points. Query perturbation data points having dimensions representing measurements of different features across perturbation cell aliquots are obtained. A composite query perturbation vector is computed between measures of central tendency across the control data points and measures of central tendency across the plurality of query perturbation data points.

Systems and methods for evaluating query perturbations

The disclosure provides methods and systems for identifying a subset of compounds in a plurality of compounds. The identifying includes obtaining, for each compound, a vector including a set of elements, where each element includes a measurement of a different feature of an instance of a cell context upon exposure to the compound. The identifying includes performing the obtaining for a plurality of cell contexts, to obtain a plurality of vectors for each compound across different cell contexts. The identifying includes combining the vectors for each compound to form a combined vector for each compound, thereby forming a plurality of combined vectors representing different compounds. The identifying includes pruning the plurality of compounds to the subset of compounds based on a similarity between respective combined vectors in the plurality of combined vectors corresponding to compounds in the plurality of compounds.

Systems and methods for high throughput compound library creation

The disclosure provides methods and systems for identifying a subset of compounds in a plurality of compounds. The identifying includes obtaining, for each compound, a vector including a set of elements, where each element includes a measurement of a different feature of an instance of a cell context upon exposure to the compound. The identifying includes repeating the obtaining for a plurality of cell contexts, to obtain a plurality of vectors for each compound across different cell contexts. The identifying includes combining the vectors for each compound to form a combined vector for each compound, thereby forming a plurality of combined vectors representing different compounds. The identifying includes pruning the plurality of compounds to the subset of compounds based on a similarity between respective combined vectors in the plurality of combined vectors corresponding to compounds in the plurality of compounds.

A method for determining whether a set of perturbations discriminates over a null distribution for an on target effect against a first component of an entity is provided. The set of perturbations comprises perturbations of the first component and the entity comprises components including the first component. There is obtained, for each respective perturbation, a corresponding vector, thereby obtaining a plurality of vectors. Each such vector comprises a plurality of elements. Each element comprises (i) a distribution metric of a different feature, in a plurality of features, across a plurality of instances of the entity upon exposure of the plurality of instances of the entity to the respective perturbation or (ii) a distribution metric of a respective dimension reduction component computed using the plurality of features for a plurality of instances of the entity upon exposure of the plurality of instances of the entity to the respective perturbation.

Growing community of inventors

Salt Lake City, UT, United States of America

Blake C Borgeson