National Technology & Engineering Solutions of Sandia, LLC

Sandia Corporation

University of California

Sandia National Laboratories

Other

Battelle Memorial Institute

Virdia, Inc.

Blake A Simmons

Seema Singh

John Michael Gladden

Eric Bryant Cummings

Tanmoy Dutta

Jian Sun

David R Wheeler

Douglas A Loy

Chad Staiger

James R McElhanon

Kamyar Rahimian

Gregory M Jamison

Timothy M Long

Thomas Zifer

Yolanda Fintschenko

Anthe George

Supratim Datta

Jian Shi

Parthasarathi Ramakrishnan

Linda Domeier

Noppadon Sathitsuksanoh

Joshua I Park

Bradley M Holmes

Ning Sun

Harsha D Magurudeniya

Ronald Francis Renzi

Timothy J Shepodd

Ryan Wesley Davis

Tao Zhang

David Ingersoll

Steven Singer

Harvey W Blanch

Edward M Rubin

Vincent Hill

Benjamin Chiau-Pin Wu

Joanne V Volponi

Kenneth Sale

Mary Bao Tran-Gyamfi

Ken Sale

Andrew Walker

Feng Xu

Patanjali Varanasi

Aaron Socha

Maxime Bergeron

Ezinne Achinivu

Hemant Choudhary

Lalitendu Das

Rajat Sapra

Manali Sawant

Noble Woo

Zhiwei Chen

Gregory McGraw

Rafael V Davalos

Robert P Jansen

Albert Alec Talin

Alfredo M Morales

Mark D Allendorf

Paul Michael Dentinger

Philip Travisano

Robert Ward Crocker

Taek Soon Lee

Jon K Magnuson

F Patrick Doty

William R Even, Jr

Gregory J Fiechtner

James Kirby

Scott E Baker

Thomas I Wallow

Gregory Daniel Friedland

Robert W Bradshaw

Swapnil R Chhabra

Renée Shediac

Karen Lee Krafcik

Gina Marie Geiselman

Daniel E Dedrick

David W Anderson

Marion C Hunter

Kim Tran

Victor C Rucker

Michael Liszka

Karen L Havenstrite

Mood Mohan

Venkataramana R Pidatala

Kaixuan Huang

Alberto Rodriguez

Saori Amaike Campen

Nawa Raj Baral

Dylan C Chivian

Timothy Charles R Brennan

Christina A Bauer

Jinxiang Zhang

Allen Salmi

Amanda C Kohler

The present invention provides for a method to deconstruct a biomass comprising: (a) introducing one or more individual components of an ionic liquid (IL) or deep eutectic solvent (DES) to a biomass, wherein the one or more individual components, and optionally any components already present in the biomass, form an IL or DES, or mixture thereof, which solubilizes the biomass to form a solubilized biomass mixture, wherein at least one individual component is introduced to the biomass separately from any other individual component; (b) optionally introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; and, (c) optionally separating the sugar from the solubilized biomass mixture.

Use of in-situ ionic liquid (IL) and deep eutectic solvent (DES) synthesis using chemically synthesized or biomass-derived ions in the pretreatment of biomass

The present invention provides for a method to deconstruct a biomass: the method comprising: (a) ensiling a biomass to produce comprising one or more organic acids, and (b) introducing a solvent to the ensiled biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent is an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, to form a solubilized biomass mixture.

Use of ensiled biomass for increased efficiency of the pretreatment of biomass

In one aspect, the present invention provides methods for preparing a fermentation product. The methods include pre-treating a mixture of biomass and ionic liquid, wherein the ionic liquid comprises a choline cation and the biomass comprises polysaccharide and lignin. The methods further include forming hydrolysates from the introduction of glycoside hydrolase to the pre-treated mixture at conditions sufficient to produce a sugar composition mixture for fermentation steps. The present invention provides methods for loading biomass mixtures in a batch-fed process, wherein the biomass slurries can be loaded into water or a concentrated sugar composition for hydrolysate production. The methods can be performed in a one-pot process, wherein the ionic liquids are present in the mixtures throughout each step. Aspects of the invention provide compositions of sugar composition mixtures and fermentation product mixtures.

High gravity, fed-batch ionic liquid based process for deconstructing biomass

The present invention also provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent to a biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent comprises (i) a metal salt, and (ii) an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, to form a solubilized biomass mixture; (b) optionally introducing an enzyme and/or a microbe to the composition such that the enzyme and/or microbe produce a biofuel and/or chemical compound from the solubilized biomass; and, (c) optionally the biofuel and/or chemical compound is separated from the composition.

Use of metal salts and deep eutectic solvents in a process to solubilize a biomass

The present invention provides for novel protic ionic liquids (PIL) or phosphate-based ionic liquid (PBIL) useful for lignocellulosic processing described herein. The novel protic ionic liquids are capable of pretreatment of lignocellulosic biomass over a wide range of pH.

Protic or phosphate-based ionic liquids useful for lignocellulosic processing

Methods and compositions are provided for ionic liquid processing of biomass.

Ammonium based ionic liquids useful for lignocellulosic processing

The present invention provides novel and improved processes for treating a lignocellulosic biomass or technical lignin using ionic liquids to obtain lignin breakdown products and polysaccharide biomass components. Recycling of ionic liquids can be included in the methods of the invention.

Renewable aromatics from lignocellulosic lignin

The present invention provides for a method of fermenting or saccharifying a biomass comprising: (a) (i) contacting a biomass comprising a polysaccharide, and an ionic liquid (IL) to form a first solution, or (ii) providing the first solution comprising the biomass and the IL, (b) contacting the first solution and carbon dioxide such that the first solution results in a lower pH, (c) introducing (i) an enzyme capable of enzymatically to breakdown at least one bond in the polysaccharide or a breakdown product of the polysaccharide, and/or (ii) a microorganism that capable of producing the enzyme and/or fermenting the polysaccharide or a breakdown product of the polysaccharide, such that the polysaccharide is at least partially broken down and the first solution is transformed into a second solution.

Method of adjusting the pH of a pretreatment solution using carbon dioxide useful for integrating saccharification and fermentation of a biomass

The present invention provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent comprising a polyamine, or a mixture of polyamines, to a biomass to dissolve at least part of solid biomass in the solvent, wherein the polyamine is a Brønsted or Lewis base, and/or the polyamine is a hydrogen bond donor and/or acceptor; (b) optionally introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; (c) optionally separating the sugar from the solubilized biomass mixture; and (d) optionally separating the lignan from the solubilized biomass mixture.

Use of polyamines in the pretreatment of biomass

The present invention provides for a genetically modified fungal host cell capable of producing prespatane and/or epi-isozizaene comprising prespatane synthase (PPS) and/or epi-isozizaene synthase (EIZS).

Genetically modified fungal cells and methods useful for producing prespatane

The present invention provides for anhost cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.

Promoter useful for high expression of a heterologous gene of interest in

The present invention relates to methods of upgrading biomass to provide useful chemical intermediates, fuels, amino acids, nutrients, etc. In particular examples, the biomass is a by-product of ethanol production and is mainly used as high-protein feed. Described herein are methods for upgrading such biomass, such as by implementing pre-treatment conditions and by employing fermentation conditions including modified organisms.

Biochemical upgrading of high-protein biomass and grain products

Disclosed herein are lignin-derived ionic liquids and methods for preparing them. The methods include forming a reaction mixture comprising a lignin-derived starting material, a carbonyl compound, and an amine; maintaining the reaction mixture under conditions sufficient to form a lignin-derived aminophenol; and converting the lignin derived aminophenol to the lignin-derived ionic liquid. Monomeric phenols, oligomeric phenols, and polymeric phenols can be used as lignin-derived starting materials.

Conversion of lignin to ionic liquids

In one aspect, the present invention provides a method for preparing a sugar composition. The method includes: forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and wherein the ionic liquid contains a dicarboxylic acid anion and a cation. The pH of the mixture is greater than or equal to about 10, and the molar ratio of the dicarboxylic acid anion to the cation is at least about 1:2. The method further includes: maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; reducing the pH of the mixture containing the dissolved polysaccharide to at least about 7; adding at least one glycoside hydrolase to the mixture having the reduced pH.

Switchable ionic liquids for biomass pretreatment and enzymatic hydrolysis

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San Francisco, CA, United States of America

Blake A Simmons