Biotec Pharmacon Asa

Universitetet I Tromsø—norges Arktiske Universitet

Bjørn Altermark

Olav Lanes

Marit Sjo Lorentzen

Terese Solstad

Ronny Helland

Ingar Leiros

Linda Havdalen

Inger Lin Uttakleiv Ræder

Man Kumari Gurung

Arne Oskar Smalås

The present invention provides to methods for the production of neuraminic acid or derivatives thereof under alkaline conditions and to the use of neuraminate lyases showing improved characteristics under alkaline conditions in the production of neuraminic acid or its derivatives. More particularly, the present invention employs inter alia a N-acetylneuraminate lyase which has been isolated from the psychrophilic bacteriumLFI1238 and has shown to have improved characteristics under alkaline conditions.

Use of a N-acetylneuraminate lyase derived from the bacteriumin the production of neuraminic acid and derivatives thereof

The present invention provides an endonuclease I or enzymatically active fragment thereof wherein said endonuclease I has the sequence of SEQ ID No. 4 or a sequence which is at least 70% identical thereto and wherein the amino acid residue which is immediately N-terminal of the FYCGC pentapeptide motif has been substituted with a residue which is negatively charged as well as nucleic acid molecules encoding these enzymes and methods of removing contaminating polynucleotides from a sample using these enzyme.

Endonucleases

Growing community of inventors

Tromsø, Norway

Bjørn Altermark