Cytiva Bioprocess R&d Ab

Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Bjorn Noren

Jean-Luc Maloisel

Gustav Jose Rodrigo

Ola Lind

Ronnie Palmgren

Virendra Kumbhar

Tomas Bjorkman

Lars C Andersson

Per-Mikael Aberg

Karol Lacki

Helena Skoglar

Karolina Busson

Jelena Vasic

Anna Arefalk

The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R-L-N(R)-L-R, immobilized on a support, wherein Ris a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; Lis either a methylene group or a covalent bond; Ris a five- or six-membered, substituted or non-substituted ring structure; Lis either a methylene group or a covalent bond; Ris a methyl group; and wherein if Ris a hydroxyethyl group and Lis a covalent bond, Ris a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

Multimodal anion exchange matrices

The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.

Separation matrices for purification of biological particles

The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

Chromatography method

The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R-L-N(R)-L-R, immobilized on a support, wherein Ris a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; Lis either a methylene group or a covalent bond; Ris a five-or six-membered, substituted or non-substituted ring structure; Lis either a methylene group or a covalent bond; Ris a methyl group; and wherein if Ris a hydroxyethyl group and Lis a covalent bond, Ris a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilized to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine or Histidine at the position corresponding to H18 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.

Affinity chromatography matrix

The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

Growing community of inventors

Uppsala, Sweden

Bjorn Noren