Pharmacia & Upjohn Aktiebolag

Kabigen Ab

Kabi Pharmacia Aktiebolag

Bjorn Nilsson

Mathias Uhlen

Lars B Abrahmsen

Erik Holmgren

Tomas Moks

Mats Lake

Cesare Sirtori

Peter Lind

Joanna Chmielewska

Guido Franceschini

Per-ANGke Nygren

Bjorn Lowenadler

Dimer of molecular variant of apolipoprotein and processes for the

Bacterial receptor structures

A process for preparing antibodies specific for an amino acid sequence which comprises immunizing a mammal with a fused protein comprising said amino acid sequence fused to an immunogenic IgG binding protein is disclosed.

Preparation of fused proteins, antibodies and processes therefore

A process for expressing proteins in Gram(-) bacteria and providing for extracellular secretion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired protein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extracellularly secreted protein; a recombinant DNA construction comprising:

Method to export gene products to the growth medium of gram negative

A recombinant DNA fragment (Z) coding for an immunoglobulin G binding domain related to staphylococcal protein A. The methionine codon of the recombinant DNA fragment has been replaced with a codon of an amino acid residue other than methionine to enable expression of a methionine-free protein. The present invention is also directed to recombinant DNA sequence containing such recombinant DNAS fragment (Z) as well as a recombinant DNA molecule, plasmid vector and bacterial cell containing such recombinant DNAS fragment. Also disclosed is a process for cleaving a fused protein expressed in a biological system using said recombinant DNA fragment.

Construction of an IgG binding protein to facilitate downstream

Growing community of inventors

Sollentuna, Sweden

Bjorn Nilsson