Novozymes A/s

Bjarne Ronfeldt Nielsen

Allan Svendsen

Torben Vedel Borchert

Helle Outtrup

Vibeke Skovgaard Nielsen

Carsten Schau Andersen

Henrik Bisgard-Frantzen

Lisbeth Hedegaard

Jesper Vind

Henrik Pedersen

Hanne Vang Hendriksen

Torben Peter Frandsen

Lisbeth Hedegaard Hoeck

Jan Lehmbeck

Ruby Ilum Nielsen

Henrik Bisg&aring;rd-Frantzen

S&oslash;ren Kj&aelig;rulff

Torben Lauesgaard Nissen

The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Cadependency, specific activity, and solubility, in particular under production conditions.

Amylase variants

The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.

Glucoamylase variants

The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Caconcentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Caconcentrations (relative to the parent).

Alpha-amylase mutants

The invention relates to starch conversion processes using glucoamylases derived fromand related glucoamylases.

Thermostable glucoamylase

The invention relates to a variant of a parent Termamyl-like -amylase, which exhibits an alteration in at least one of the following properties relative to said parent -amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60&deg; C.

&agr;-amylase mutants

The present invention relates to isolated nucleic acid sequences encoding polypeptides having alpha-amylase activity &lsqb;E.C. 3.2.1.1&rsqb;, which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Nucleic acid sequences encoding alkaline alpha-amylases

The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca concentrations (relative to the parent).

&agr;-amylase and &agr;-amylase variants

The present invention relates to isolated polypeptides having &agr;-amylase activity and isolated nucleic acid sequences encoding the polypeptides, which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Growing community of inventors

Virum, Denmark

Bjarne Ronfeldt Nielsen