Leland Stanford Junior University

Bita Sahaf

Leonore A Herzenberg

David Miklos

Lenore A Herzenberg

The described invention comprises a method for detecting the onset or monitoring the recurrence of chronic graft versus host disease (cGVHD) in a transplantation patient. The method comprises isolating peripheral blood mononuclear cells (PBMCs) from the transplantation patient and analyzing the isolated PBMCs to detect a biomarker specific for a donor cell. The detection of the biomarker is indicative of the onset or recurrence of cGVHD in the transplantation patient.

Method to detect the onset and to monitor the recurrence of chronic graft versus host disease in transplantation patients

The present invention relates to methods to activate peripheral blood mononuclear cells. The present invention further provides methods for the identification of blocking agents of Tat protein activation of PBMCs and for identifying an active portion of a Tat polypeptide for incorporation as an immunogen in a vaccine. The present invention also provides an immunogen comprising an isolated variant of wild-type HIV Tat protein that exhibits reduced HIV priming of competent cells in comparison to the wild-type HIV Tat protein. The present invention further provides a diagnostic kit for identifying blocking agents for HIV Tat protein.

Methods for activating peripheral blood mononuclear cells (PBMCs) by administering human immunodeficiency virus (HIV) Tat under physiological oxygen levels

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Palo Alto, CA, United States of America

Bita Sahaf