Diagnostic Biosystems

Other

Marc Key

Bipin Gupta

Marc E Key

An antigen protection method for enabling delayed sample processing may include: fixing a sample; embedding the sample; sectioning the sample and placing a section of the sample in contact with a microscope slide to produce a sectioned sample; de-embedding the sectioned sample; optionally rehydrating the sectioned sample; performing target retrieval on the sectioned sample; and optionally performing target visualization on the sectioned sample. The method may further include the steps of: applying a protecting reagent onto the sectioned sample; drying the protecting reagent; and waiting a period of time, such as greater than six hours, and these steps may be performed after the de-embedding step, rehydrating step, and/or target retrieval step.

Antigen protection method for enabling delayed sample processing

A sample protection method is provided which may be used for protecting a biological sample on a microscope slide, such as during heat-induced target retrieval and/or after heat-induced target retrieval such that: 1) the sample remains adherent to the microscope slide; and 2) the microscopic morphology of the biological sample remains intact. In some embodiments, the sample protection method may include the steps of: creating a sectioned sample that is in contact with a microscope slide; applying a protecting reagent onto a sectioned sample that is in contact with a microscope slide and drying the protecting reagent in which the protecting reagent may be both applied and dried onto the sectioned sample before and/or after performing target retrieval on the sectioned sample. The protecting reagent may include a water-soluble polymer and/or a water-soluble wax, such as polyethylene glycol, polypropylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, alginic acid, and carrageenan.

Sample protection method

A deparaffinizing composition and method are provided for removing paraffin wax from biological samples. The deparaffinizing composition may include: a paraffin solvent that may be a first apolar paraffin solvent; a second apolar solvent that may be at least partially miscible with water; a first polar solvent that may be miscible with both water and apolar solvents; a second polar solvent that may be water; and a detergent. The deparaffinizing method may include the steps of: incubating the sample for a time period in a deparaffinizing composition; and rinsing the sample with an aqueous buffer. Preferably, the step of incubating the sample for a time period in the deparaffinizing composition may include heating the sample and the composition to between approximately 35 to 65 degrees Celsius.

Deparaffinizing compositions and methods

The present disclosure relates to counterstains for staining a biological sample on a single slide in preparation for microscopic examination. The counterstains are used to analyze the sample on the single slide using both brightfield and fluorescent illumination. The counterstains can identify both morphological details and molecular structures within the cells contained in the sample. The counterstains can be used in conjunction with other molecular stains.

Counterstains for a biological sample

The present disclosure relates to an aqueous sealing fluid for sealing a stained biological specimen present on a microscope slide for microscopic analysis. The sealing fluid protects the biological specimen from drying artifacts, protects stains performed on biological specimen from fading, and protects stains performed on biological specimens from being dissolved by organic mounting media. Methods of using the aqueous sealing fluid are also disclosed.

Sealing fluid for microfluidic analyses

A method for labeling concentration density differentials of an analyte in a biological sample is provided. The method may including the steps of: binding an enzyme to an analyte contained in a sample, the enzyme capable of acting on at least two chromogens; incubating the sample with a first chromogen for a first time period to generate a first color chromogen-enzyme product, the first color chromogen-enzyme product reflecting light observable as a first color; and incubating the sample with a second chromogen for a second time period to generate a second color chromogen-enzyme product, the second color chromogen-enzyme product reflecting light observable as second color. A combination of the light observable as the first color and the light observable as the second color may be observable as a third color, and each color may describe a different analyte density in the biological sample.

Method for labeling concentration density differentials of an analyte in a biological sample

The present disclosure relates methods of chromogen layering, wherein a first chromogen and a first stain (color) are produced on a sample, specific for a first analyte, followed by a second chromogen and a second stain (color) being produced on the same sample, specific for a second analyte. In addition, if desired, by overlaying the second stain on top of the first stain, a unique third color is produced that is specific for a third analyte. Therefore, the distribution of different colors throughout the sample could be used to identify at least two or more analytes simultaneously within a single sample.

Chromogen layering for color generation

Disclosed are methods for treating a biological sample for an analyte-staining assay. These methods are conducted with the use of a single aqueous multi-functional composition for the purposes of performing the steps of deparaffinization, rehydration, antigen retrieval and endogenous enzyme blocking simultaneously optionally at an elevated temperature. These methods are alternatively conducted with use of a single aqueous multi-functional composition that contains a thermostable protease for the purpose of deparaffinization, rehydration, antigen retrieval and endogenous enzyme blocking at an elevated temperature.

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Pleasanton, CA, United States of America

Bipin Gupta