Other

Petrochina Company Limited

Chinese Academy of Agricultural Sciences

Feed Research Institute Chinese Academy of Agricut

Institute of Animal Science of Chinese Academy

Feed Research Institute, Chinese Academy of Agricu

Bin Yao

Wei Wang

Jie Zhang

Huoqing Huang

Yaru Wang

Huiying Luo

Yingguo Bai

Xiaoyun Su

Kun Meng

Yuan Wang

Tao Tu

Peilong Yang

Rui Ma

Hongjun Lu

Yong Wang

Lijun Mu

Xianwen Li

Zhaojie Song

Lei Sui

Xiaobing Lu

Suiwang Zhang

Erzhen Wang

Zhiying Deng

Zhenning Ji

Pengjun Shi

Xia Shi

Zijian Wang

Weina Liu

Fuwei Bi

Canfang Niu

Yangyang Li

Yafeng Ju

Jiuzheng Yu

Lingzhi Yang

Hong Yang

Fei Gao

Wei Xia

Yuan Gu

Jin Qiu

Yanqing Liu

Yujie Guo

Loulou Li

Juntao Wang

Jianzhong Ge

Yeqing Li

Bilin Luo

Chao Shao

Heng Zhao

Tiezheng Yuan

Xiuyun Shi

Huang Qin

Yongkai Du

Jianshuang Ma

Dali Yu

Gaixing Hu

Xiao Jiang

The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein andcell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of, so as to construct a strain displaying red fuorescent protein on the surface of. By sortingstrains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

Recombinant expression vector applicable to rapid screening for recombinant strain and application

The present invention relates to the field of genetic engineering, particularly to a glucoamylase TIGa15, gene and application thereof. Said glucoamylase comprises the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2 and has the excellent enzymic properties, which can be applied to feed, food, and medicine industries, can be industrially produce with the genetic engineering technics.

Glucoamylase TLGA15 and gene and application thereof

The present invention provides use of a manganese peroxidase in the detoxification of mycotoxins, and specifically, the present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6), genes thereof, and uses thereof. The present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6) derived from lignocellulose degradation bacteria, the amino acid sequences thereof being as set forth in SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 10, and SEQ ID NO: 13.

Manganese peroxidase, gene thereof, and use thereof in detoxification of mycotoxin

The present invention takes the glucose oxidase GOD fromas the mutation template to obtain the glucose oxidase GOD mutants with improved catalytic efficiency and thermal stability by site directed mutagenesis. The specific activity of the mutant of the present invention is 66% higher than that of the wild type GOD; the enzyme activity of the mutant of the present invention is 13.6 times higher than that of the wild type after being treated at 70&#xb0; C. for 10 min; and the enzyme activity of the mutant of the present invention is 29.4 times higher than that of the wild type after being treated at 80&#xb0; C. for 2 min.

Glucose oxidase GOD mutant and gene and application thereof

The present invention relates to a mannanase PMan5A mutant having improved heat resistance, a gene encoding the mutant and application thereof. The mutant is obtained by a substitution of histidine with tyrosine at amino acid residue 93, phenylalanine with tyrosine at amino acid residue 94, leucine with histidine at amino acid residue 356, and/or alanine with proline at amino acid residue 389. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild-type mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play an important role for the thermal stability of the mannanase of GH5 family.

Mannanase PMAN5A mutant having improved heat resistance, gene thereof, and application

The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.

Amylase mutant having high specific activity and thermal stability, gene of mutant, and applications thereof

The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162site of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230site of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry.

Phytase variants YeAPPA having improved gastric protein resistance and acid resistance, and increased catalytic efficiency

The present invention relates to the field of genetic engineering, particularly to phytase variants YkAPPA having amino acid sequence substituting Leucine at the 162site of the sequence set forth in SEQ ID NO.1 with glycine or proline, or having amino acid sequence substituting glutamic acid at the 230site of the sequence set forth in SEQ ID NO.1 with glycine, alanine, serine, threonine, aspartic acid, proline, or arginine, and having improved pepsin resistance and increased catalytic efficiency of 2.1 times of that of the wild phytase, in the benefit of the development of economical feed enzyme industry.

Phytase variants YkAPPA having improved pepsin resistance and increased catalytic efficiency

The present invention provides a stratified waterflooding apparatus, which comprises: a water distribution unit, which comprises two water distributors, the water distributor having an inner side wall and an outer side wall, the inner and outer side walls forming a first annular cavity therebetween and being respectively provided with a water inlet and a water outlet which are communicated with the first annular cavity, the first annular cavity accommodates a driving mechanism and an adjustable blocking member, the adjustable blocking member having a first operating position in which it blocks the water inlet or the water outlet and a second operating position in which it releases the blocking of the water inlet and the water outlet; and a controller in communication connection with the driving mechanism. The stratified waterflooding apparatus can realize intelligent and automatic regulation of an aperture of the water distributors in the stratified waterflooding process.

Stratified waterflooding apparatus

An injection stimulation agent for water injection well and the preparation method and application thereof are described. The raw material composition of the injection stimulation agent for water injection well comprises: 5.0%-10.0% of sulfamic acid, 10.0%-20.0% of methanol, 10.0%-20.0% of tartaric acid, 10.0%-20.0% of ethylenediamine tetramethylene phosphoric acid, 5.0%-10.0% of sodium polyacrylate, 5.0%-8.0% of cocoyl hydroxyethyl sulfonic acid and the balance of water. Embodiments include a preparation method of the above injection stimulation agent. The injection stimulation agent for water injection well can be used in acidizing treatment process of the reservoir, and can be used directly. The injection stimulation agent can play the role of shrinking swelled clay at the same time of dissolving scale, and finally achieve the purpose of depressurization and enhanced production.

Injection stimulation agent for water injection well and the preparation method and application thereof

On-line diverting acids for continuous injection into water injection wells and methods of preparing the diverting acid are disclosed. The on-line diverting acids have a raw material composition of: 5.0-25.0% of hydrochloric acid, 3.0%-6.0% of ammonium fluoride, 4.0%-8.0% of a diverting agent, 3.0%-7.0% of a chelating agent, 1.0-3.0% of a corrosion inhibitor, 1.0-3.0% of an anti-swelling/swelled-clay-shrinking agent and a balance of water. The on-line diverting acids can remove plugging from deep zones of high and low permeability layers, and can be continuously injected to simplify the construction process, to achieve the purpose of reducing the pressure and stimulating the injection.

On-line diverting acid for continuous injection into water injection wells and a preparation method thereof

Provided are acidic thermophilic polygalacturonase TePG28A, encoding gene and application thereof. The amino acid sequence thereof is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The expressed acidic thermophilic polygalacturonase by means of cloning has advantages such as high enzyme activity and high stability; can adapt to the high temperature environment in the industrial production; has better application prospect; and can effectively degrade pectic substances such as polygalacturonic acid and pectin; and can be effectively applied to the industrial field of feed, food, and textile, etc.

Acidic thermophilic polygalacturonase TEPG28A, and encoding gene and application thereof

Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO:1 or SEQ ID NO:2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.

Glucose oxidase CnGODA and gene and application thereof

A layered water injection system and a layered water injection method. The system comprises: a ground water injection valve group configured with an intelligent control valve, for receiving a water injection control instruction sent by a remote control center, and for converting the water injection control instruction into a switching action of the intelligent control valve to control water flow pressure fluctuation in a water injection string, so as to form a first pressure wave code in the water injection string; a water distributor for collecting the first pressure wave code, interpreting the first pressure wave code as a corresponding water outlet switch instruction, and performing layered water injection control according to the water outlet switch instruction. The embodiments of the application can improve the automation level of layered water injection and the reliability of layered water injection control.

Layered water injection system and layered water injection method

A chelating agent may be used for reservoir acidification and stimulating injection. The raw material composition of the chelating agent comprises: 10.0%-20.0% of methanesulfonic acid, 10.0%-20.0% of methanol, 10.0%-20.0% of polyaspartic acid, 10.0%-15.0% of epoxy succinic acid, 5.0%-10.0% of sodium cetyl sulfonate, 10.0%-15.0% of cetyltrimethylammonium bromide and the balance of water. The present disclosure also provides a preparation method of the above chelating agent. The chelating agent for reservoir acidification and stimulating injection may be used in an acidizing and stimulating injection process, wherein it can effectively dissolve rock cracks, be used directly without need to be reversed.

Chelating agent for reservoir acidification and stimulating injection and its preparation method and application

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Beijing, China

Bin Yao