Sigma-aldrich Co. LLC

Eastman-kodak Company

Billy L Brizzard

Douglas Lincoln Vizard

Richard George Chubet

Ron Hernan

Darlene W Bianca

Thomas Patrick Hopp

The present invention provides novel identification polypeptides containing multiple copies of an antigenic domain joined in tandem to provide increased sensitivity for the detection and purification of target peptides, a cleavable linking sequence and optionally a spacer domain. Further provided are hybrid polypeptide molecules composed of an identification polypeptide and a target peptide which are produced by recombinant DNA technology and purified using affinity chromatography using one or more ligands. Accordingly, also provided are DNA expression vectors containing DNA encoding for identification polypeptides and methods for using such identification polypeptides for the purification of target peptides. Also provided are methods of constructing DNA vectors encoding the novel identification polypeptides and DNA expression vectors encoding the identification polypeptides linked to a target peptide.

Purification of recombinant proteins fused to multiple epitopes

This invention discloses a gene for the identification of cells comprising a secretion leader segment, a cell marker segment and a transmembrane segment. The gene can be used to identify cells transfected with the gene by the steps of: inserting the gene having a secretion leader segment, a cell marker segment and a transmembrane segment into the DNA or RNA of a cell, allowing the cell to express the gene, and detecting the expressed cell marker segment of the gene.

Method of identifying cells with polypeptide surface marker

This invention discloses a gene for the identification of cells comprising a selection leader segment, a cell marker segment and a transmembrane segment. The gene can be used to identify cells transfected with the gene by the steps of: inserting the gene having a selection leader segment, a cell marker segment and a transmembrane segment into the DNA or RNA of a cell, allowing the cell to express the gene, and detecting the expressed cell marker segment of the gene.

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New Haven, CT, United States of America

Billy L Brizzard