10x Genomics, Inc.

Bill Kengli Lin

Rajiv Bharadwaj

Anthony Makarewicz

Tobias Daniel Wheeler

Mohammad Rahimi Lenji

Michael Schnall-Levin

Christopher Hindson

Pranav Patel

Andrew D Price

Yifeng Yin

Katherine Pfeiffer

Zahra Kamila Belhocine

Augusto Manuel Tentori

Hanyoup Kim

Felice Alessio Bava

Siyuan Xing

Pratomo Alimsijah

Nabil Mikhaiel

Denis Pristinski

Kamila Belhocine

Cedric Uytingco

Alexander Post Kindwall

Kevin Gilmartin

Jill Herschleb

Michael John Tierney

Eric Anderson

Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

Fluid delivery methods

Provided herein are materials and methods for in situ labeling of a biological sample for use in spatial analysis of one or more analytes in the biological sample. In some aspects, in situ labeling of a biological sample includes labeling the biological sample with one or more spatial barcodes.

In situ spatial barcoding

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

Droplet forming devices and system with differential surface properties

Devices and methods are provided for reducing or removing condensation from compressed air.

Condensation removal from compressed gas systems

Provided are methods of capturing an analyte from a biological sample using a hydrogel that includes capture probes to capture the analyte, identifying a region of interest of the biological sample, and isolating the region of interest from the biological sample by removing a portion of the hydrogel that corresponds to the region of interest. Compositions and kits for performing the methods are also provided.

Spatial analysis utilizing degradable hydrogels

Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Microfluidic channel networks for partitioning

Method of producing emulsions

Devices, systems, and kits for forming droplets

Devices having a plurality of droplet formation regions

Growing community of inventors

Pleasanton, CA, United States of America

Bill Kengli Lin