Medtronic Minimed, Inc.

Minimed, Inc.

Bill C Ponder

David J Vachon

Glenn Noronha

Brooks B Cochran

Aaron M Heiss

Joseph C Walsh

Jonathan Reilly

William P Van Antwerp

Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. These biosensor molecules address the problem of obtaining fluorescence emission at wavelengths greater than about 500 nm. Biosensor molecules generally include an (1) an acridine-based fluorophore, (2) a linker moiety and (3) a boronate substrate recognition/binding moiety, which binds polyhydroxylate analytes, such as glucose. These biosensor molecules further include a 'switch' element that is drawn from the electronic interactions among these submolecular components. This fluorescent switch is generally 'off' in the absence of bound polyhydroxylate analyte and is generally &#x201c;on&#x201d; in the presence of bound polyhydroxylate analyte. Thus, the reversible binding of a polyhydroxylate analyte essentially turns the fluorescent switch &#x201c;on&#x201d; and &#x201c;off&#x201d;. This property of the biosensor molecules, as well as their ability to emit fluorescent light at greater than about 500 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

Analyte sensing via acridine-based boronate biosensors

Improved polymer matrices which incorporate fluorescent biosensor molecules as well as methods of making and using these polymer matrices are described. Such matrices can be used in fluorescent biosensors and biosensor systems, including those which are used in the detection of polyhydroxylated analytes such as glucose. The properties of the polymer matrices of the invention renders biosensors utilizing such matrices particularly well-suited for detecting and measuring in-vivo glucose concentrations.

Polymers functionalized with fluorescent boronate motifs and methods for making them

Disclosed is a biocompatible membrane comprising a hydrophilic polyurea composition. The hydrophilic polyurea composition comprises the product of a reaction mixture comprising (a) an amino terminated polysiloxane, (b) a hydrophilic polymer selected from the group consisting of a diamino terminated copolymer of polypropylene glycol and polyethylene glycol, polyethylene glycol, polypropylene glycol and diamino polyethylene glycol having an average molecular weight of from about 400 to about 2000, and (c) a diisocyanate selected from the group consisting of hexamethylene-1,6-diisocyanate, dicyclohexylmethane 4,4&prime;-diisocyanate, and isophorone diisocyanate, and constituting about 50 mole % of the reaction mixture. In this mixture, (a) and (b) constitute a polymeric portion of the reaction mixture, and the hydrophilic polyurea composition has a ratio of its diffusion coefficient for oxygen to its diffusion coefficient for glucose of from about 2,000 to about 10,000. Also provided are biosensors coated with a membrane of the invention, and methods of using such biosensors to measure an analyte in a tissue of a subject.

Hydrophilic polymeric material for coating biosensors

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Fort Worth, TX, United States of America

Bill C Ponder