National Taiwan University

Sun, Chi-kuang

Bhaskar Jyoti Borah

Chi-Kuang Sun

Chi-kuang Sun

The present disclosure relates to a data processing method, and more specifically, to a digital image processing method to enable a rapid noise-suppressed contrast enhancement in an optical linear or nonlinear microscopy imaging application. The disclosed method digitally mimics a hardware-based feedback-driven adaptive or controlled illumination technique by means of digitally resembling selective laser-on and laser-off states so as to selectively optimize the signal strength and hence the visibility of the weak-intensity morphologies while mostly preventing saturation of the brightest structures.

GPU-accelerated data processing method for rapid noise-suppressed contrast enhancement

A data processing method for rapidly suppressing background high frequency noise in a digitized image. The data processing method includes configuring a graphical processing unit to perform a first amplification process, a pixel binning process or a first interpolation process, a first low-pass filtering process, a second interpolation process, a first subtraction process, a second low-pass filtering process, a second amplification process, and a second subtraction process on an input image, so as to subtract a subtraction mask from the input image and generate a noise-suppressed output image.

Data processing method for rapidly suppressing high-frequency background noise in a digitized image

The field of view (FOV) of a nonlinear optical microscope (NLOM) is expected to be large enough for employing high-speed raster scanning on a mesoscale volumetric biological sample. Concurrently, three-dimensional (3D) visualization of fine sub-micron biological structures requires high enough lateral and axial resolutions, enforcing a high numerical aperture (NA) objective lens to be employed, thereby limiting the FOV of an NLOM. The invention is directed to a laser scanning NLOM, or to a large-angle optical raster scanning system, for deep biological tissue imaging with a large FOV of more than one square millimeter, up to 1.6&#xd7;1.6 mm, while simultaneously maintaining a sub-femtoliter effective 3D resolution by means of a high-NA and low magnification objective lens and further maintaining a high acquisition speed with synchronized sampling, limited by the repetition rate of a high repetition rate pulsed laser source, thereby exceeding Nyquist Criterion for resolving micro-optical resolution throughout a horizontal FOV of more than one millimeter.

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Taipei, Taiwan

Bhaskar Jyoti Borah