Ge Healthcare Bio-sciences Ab

Cytiva Sweden Ab

Bharath Subrahmanya

Stefan Sjolander

Hakan Erik Roos

Per Kjellin

Girish Kittur

Mahesh Bhat

Lars Erik Hammarstrand

Kalyana Chakravarthy Duggirala

Urban Jonsson Axelsson

Shekar Ambepu

A self-adhesive layered septum is disclosed. In one example, the septum includes a first outer layer including a thermoplastic elastomer such as a styrenic block copolymer, containing styrene ethylene butylene styrene (TPE-SEBS) capable of closing at least partially an aperture formed when a needle is inserted through the layer; an adhesive second layer for adhering the septum to a mouth area of a well or container to which the septum is attachable; and a thermoplastic third layer between the first and second layers, thermobonded to the first layer and providing better adherence for the adhesive layer. The first layer includes a recess and a vent, which reduce pressure differentials in use, but together with the third layer minimize evaporation through the septum.

Septa

An integrated system for performing electro-blotting, probing and drying of the membrane is disclosed. The integrated system comprises a transfer unit for receiving one or more transfer sandwich holder. Each transfer sandwich holder holding a transfer sandwich comprises a gel member and the membrane. The transfer unit is configured to transfer samples from the gel member to the membrane. The integrated system also includes a probing unit for receiving the membrane therewithin. The membrane is exposed to a plurality of antibodies for binding with the samples in the membrane. A drying unit is also present for drying the membrane with hot air.

Integrated system for electro-blotting

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Bengaluru, India

Bharath Subrahmanya