Somalogic, Incorporated

Quest Diagnostics Investments, Inc.

Somalogic Operating Co., Inc.

Bharat Nathu Gawande

Nebojsa Janjic

Daniel J Schneider

Malti P Nikrad

Jeff Carter

John C Rohloff

Weimin Sun

Charles M Strom

Chris Bock

Deborah Ayers

Jennifer C Bertino

Noh Jin Park

John Rohloff

Polynucleotides, such as aptamers, comprising at least first one 5-position modified pyrimidine and at least one second 5-position modified pyrimidine are provided, wherein the first and second 5-position modified pyrimidines are different. Methods of selecting and using such polynucleotides, such as aptamers, are also provided.

Oligonucleotides comprising modified nucleosides

The present invention provides aptamers that specifically bind to the EGF receptor in a sample, and diagnostic and analytical methods using those aptamers. In some embodiments, the aptamers include a 3' cap. In some embodiments, the 3&#x2032; cap is an inverted deoxythymidine. In some embodiments the aptamers include a spacer and at least one moiety selected from the group consisting of binding pair member and a detectable label, wherein the spacer is attached to the 5&#x2032;-end of the aptamer and the moiety is attached the 5&#x2032; end of the spacer. In some embodiments the spacer is hexaethylene glycol. In some embodiments, the binding pair member biotin. In some embodiments the detectable label is a fluorophore.

Aptamers and diagnostic methods for detecting the EGF receptor

Described herein are 5-position modified cytosine nucleotides and nucleosides as well as phosphoramidites and triphosphates derivatives thereof. Further provided are methods of making and using the same, and compositions and uses of the modified nucleosides as part of a nucleic acid molecule (e.g., aptamer).

Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto

The present invention provides aptamers that specifically bind to the EGF receptor in a sample, and diagnostic and analytical methods using those aptamers.

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Lafayette, CO, United States of America

Bharat Nathu Gawande