Handylab, Inc.

Other

Betty Wu

Kalyan Handique

Gene Parunak

Karthik Ganesan

Sundaresh N Brahmasandra

Nikhil Phadke

Aaron Kehrer

Jeff Williams

John S Althaus

Cecelia Haley

Ted Springer

This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Integrated system for processing microfluidic samples, and method of using same

A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Processing particle-containing samples

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.

Method for processing polynucleotide-containing samples

Integrated system for processing microfluidic samples, and method of using the same

System for processing polynucleotide-containing samples

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Canton, MI, United States of America

Betty Wu