Degussa Aktiengesellschaft

Evonik Degussa Gmbh

Degussa - Huls Ag

Other

Bettina Mockel

Walter Pfefferle

Hermann Sahm

Lothar Eggeling

Achim Marx

Kevin Burke

Ashling McCormack

Miroslav Patek

Mechthild Rieping

Caroline Kreutzer

Stephan Hans

Cliona Stapelton

Laurence Kieran Dunican

Mike Farwick

Christian Riedel

Caroline Kruetzer

Sahm Hermann

Brigitte Bathe

Georg Thierbach

Thomas Hermann

Bernhard Eikmanns

Alfred P&uuml;hler

Anke Weissenborn

Michael Hartmann

Bernd Eikmanns

J&ouml;rn Kalinowski

Yvonne Tilg

Rita Dunican, Legal Representative

Albert De Graaf

Michael O'Donohue

L K Dunican

Cliona Stapleton

Bernard Eikmanns

This application discloses methods for the preparation of L-amino acids, which comprises fermentation of a desired L-amino acid-producing bacteria in which at least the tal gene is amplified. In some embodiments, genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified.

Process for the preparation of L-amino acids

L-lysine-producing strains ofwith enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and a process for the preparation of L-lysine.

L-lysine-producing corynebacteria and process for the preparation of L-lysine

The invention is directed to DNA sequences from coryneform bacteria, particularly, which encode a protein having transaldolase enzymatic activity. The invention also encompasses processes for the fermentative production of L-amino acids using bacteria in which a gene encoding transaldolase is amplified.

Process for the preparation of L-amino acids by overexpressing the tal gene encoding transaldolase

The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

The invention related to an isolated nucleic acid consisting of a fragment of the polynucleotide ofcitA gene or a fragment of the full complement of the polynucleotide of thecitA gene wherein said fragment is at least 30 consecutive nucleotide in length, and the vector and host cell harboring these nucleic acids.

[object Object]

The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.

Coryneform bacteria which produce chemical compounds I

The invention relates to isolated nucleotide sequences from Coryneform bacteria which code for the pck gene encoding the enzyme phosphoenol pyruvate carboxykinase (PEP carboxykinase). The invention also relates a process for the fermentative preparation of L-amino acids, in particular L-lysine, L-threonine, and L-glutamate by attenuation of the pck gene.

Nucleotide sequences which code for the pck gene

The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

L-lysine-producing corynebacteria and process for the preparation of lysine

Nucleotide sequences encoding the ptsH gene

The invention is directed to DNA sequences from coryneform bacteria which encode a protein having transaldolase enzymatic activity. The invention also encompasses methods for the fermentative production of L-amino acids using bacteria in which the gene encoding transaldolase is amplified.

Nucleotide sequences which code for the tal gene

Nucleotide sequences coding for the glbO gene

The invention relates to an isolated polynucleotide from coryneform bacteria wherein the polynucleotide encodes a protein having n-succinylaminokeptopimelate transaminase activity.

Nucleotide sequences encoding the dapC gene and process for the production of L-lysine

The present invention is directed to isolated polynucleotides coding for phosphoglucose isomerase (pgi) from coryneform bacteria. In addition, the invention includes methods for increasing the metabolic flux through pentose phosphate cycle of bacteria by reducing or eliminating the activity of pgi. These methods may be used to increase the fermentative production of nucleotides, vitamins and amino acids.

Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)

Growing community of inventors

Bielefeld, Germany

Bettina Mockel