University of Texas System

Bethany Ann Janowski

David R Corey

John Dorrance Minna

David S Shames

David John Mangelsdorf

David Reid Corey

John D Minna

Transcription of a gene in a mammalian cell is methylase-independently inhibited by contacting the cell with a nucleic acid oligomer of 12-28 bases complementary for a partially single-stranded target genomic sequence of the gene.

Antigene oligomers inhibit transcription

Synthesis of a target transcript of a gene is selectively increased in a mammalian cell by contacting the cell with a polynucleotide oligomer of 12-28 bases complementary to a region within a target promoter of the gene under conditions whereby the oligomer selectively increases synthesis of the target transcript.

Modulation of gene expression by oligomers targeted to chromosomal DNA

The present invention provides a method of screening for agonists of an oxycholesterol activator of LXR&#x3b1; transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; treating the host cell with potential LXR-specific ligands; and identifying compounds which activate LXR&#x3b1; transcription. Also provided is a method of screening for antagonists of an oxycholesterol activator of LXR&#x3b1; transcription, comprising the steps of: introducing a reporter construct and an LXR expression construct into a host cell; pretreating the host cell with activators of LXR&#x3b1; transcription; contacting the host cell with potential antagonists of LXR&#x3b1; transcription; and identifying compounds which block the activation the LXR&#x3b1; transcription.

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Dallas, TX, United States of America

Bethany Ann Janowski