Archer Daniels Midland Company

Beth Fatland-Bloom

P John Rayapati

Nyerhovwo John Tonukari

Gyan P Rai

Sean Jordan

Lhing-Yew Li

John P Rayapati

John Rayapati

Disclosed herein is a newly discovered problem and solution for engineeringto ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. The problem is that when grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase fromwhich is less inhibited by xylitol than other xylose isomerases, exemplified byxylose isomerase. Expression of thexylose isomerase may be used alone, or in combination with expression of a xylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.

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Methods of producing C4 dicarboxylic acids are disclosed. Nucleotide sequences encoding pyruvate carboxylase and uses of such nucleotide sequences in the production of C4 dicarboxylic acids are further disclosed.

Methods of producing C4 dicarboxylic acids

Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as acell, are provided. Methods of manipulating carbon flux in a cell also are provided.

Compositions and methods for manipulating carbon flux in cells

Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as acell, are provided. Methods of manipulating carbon flux in a cell also are provided.

Growing community of inventors

Decatur, IL, United States of America

Beth Fatland-Bloom