Gist-brocades N.v.

Gist-brocades B.v.

Dsm IP Assets B.v.

Dsm N.v.

Bertus Pieter Koekman

Roelof Ary Bovenberg

Jan Metske Van Der Laan

Masako Shinjoh

Jan Verweij

Andreas Hoekema

Pieter Van Solingen

Nigel John Mouncey

Erik De Vroom

Annemarie Eveline Veenstra

Dirk Schipper

Adrianus Wilhelmus Vollebregt

Dick Schipper

Connie Lee

Markus G Goese

Christine Toepfer

Corina Hauk

Anne F Mayer

Anja Meury

Adrianus W H Vollebregt

Marina Van Gelder

Marie-Gabrielle Beuzelin-Ollivier

Manuela Dalluege

Bastien Chevreux

Juan F Martin

Lucia Helena Van Der Voort

Bastian Chevreux

Jose Luis Barredo

Petrus Wilhelmus Van Dijck

Bina Jamnadas Mehta

Enrique Cerda-Olmedo

Martien A Groenen

Santiago Gutierrez

Bruno Diez

Emilio Alvarez

Martinus A Groenen

Christina Esmahan

Martien Am Groenen

Petrus W Van Dijck

Bina J Mehta

The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

Fermentative Vitamin C production

Fermentative vitamin C production

Process for the production of 7-ADCA via expandase activity on

Process for the efficient production of 7-ADCA via 3-(carboxyethylthio)

An overall process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum transformant strain expressing expandase.

Process for the efficient production of 7-ADCA via

Intersexual heterozygous phycomyces

Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the antibiotic. The invention is exemplified with improved production of penicillin in Penicillium chrysogenum, with the isolation of another clustered biosynthetic gene(s) and with the expression of penicillin biosynthetic genes in Acremonium chrysogenum.

Method and compositions for enhancing production of secondary

Novel methods and compositions are provided for the enhanced production of secondary metabolites. Methods are provided for identifying sequences which when transformed into secondary metabolite producing hosts, enhance the production of secondary metabolite. The process is exemplified for penicillin. In addition, the P. chrysogenum acyltransferase gene is isolated and sequenced.

Method for identifying and using biosynthetic genes for enhanced

Growing community of inventors

Schipluiden, Netherlands

Bertus Pieter Koekman