Meristem Therapeutics S.a.

Other

Institut National De La Sante Et De La Recherche Medicale

Edulis

Meristern Therapeutics

Bertrand Merot

Veronique Gruber

Philippe Bournat

Philippe Lenee

Valerie Salmon

Sylvie Baudino

Genevieve Spik

Dominique Legrand

Michael Marden

Claude Poyart

Renee-Josee Pagnier

Wilfrid Dieryck

Claude Benicourt

Florence Ruggiero

V&eacute;ronique Gruber

Claire Cudrey

Jean-Yves Exposito

Robert Garrone

Jeanne Comte

The invention concerns the use of a recombinant nucleotide sequence containing a cDNA coding for a lactoferrin, in particular human lactoferrin, or the derived proteins, and elements enabling a plant cell to produce lactoferrin or the derived proteins, coded by the cDNA, in particular a transcription promoter and terminator identified by the plant cell transcription machinery, to transform plant cells in order to obtain, from these cells, or plants obtained therefrom, lactoferrin or derived proteins.

Recombinant lactoferrins, methods of production from plants and uses thereof

The invention concerns the use of a recombinant nucleotide sequence containing a cDNA coding for a lactoferrin, in particular human lactoferrin, or the derived proteins, and elements enabling a plant cell to produce lactoferrin or the derived proteins, coded by said cDNA, in particular a transcription promoter and terminator identified by the plant cell transcription machinery, to transform plant cells in order to obtain, from these cells, or plants obtained therefrom, lactoferrin or derived proteins.

A method for producing haemin proteins by (i) inserting into plant cells one or more nucleic acid molecules that each comprise at least one sequence coding for a protein component of an animal haemin protein capable of reversibly binding oxygen, or for a variant or portion of said protein component, and optionally a sequence coding for a selection agent; (ii) selecting cells containing nucleic acid coding for the protein component of the haemin protein; (iii) optionally propagating the transformed cells either in a culture or by regenerating whole transgenic or chimeric plants; and (iv) recovering and optionally purifying a haemin protein that includes a complex consisting of the protein or proteins coded for by said nucleic acid and at least one iron-containing porphyritic nucleus, or a plurality of such complexes.

Method for producing hemin proteins using plant cells, resulting proteins and products containing same

The invention concerns the use of a recombinant nucleotide sequence containing a cDNA coding for one or several mammalian collagen chains or derived proteins and elements enabling a plant cell to produce the collagen chains(s) or derived proteins, coded by said cDNA, particularly a transcription promoter and terminator identified by the transcription machinery of the plant cells, for transforming the plant cells so as to obtain from these cells, or plants obtained from them, the collagen chain(s) or derived proteins. The invention also concerns the resulting proteins and transformed plant material as well as their uses.

Recombinant collagen and derived proteins produced by plants, methods for obtaining them and uses

Recombinant preduodenal lipases and polypeptides derivatives produced by plants, processes for obtaining them and their uses

Recombinant lactoferrin, methods of production from plants and uses

A method for producing human hemoglobin proteins by (i) inserting into plant cells one or more nucleic acid molecules that each comprise at least one sequence coding for a protein component of a human hemoglobin protein capable of reversibly binding oxygen, and optionally a sequence coding for a selection agent; (ii) selecting cells containing nucleic acid coding for the protein component of the human hemoglobin protein; (iii) optionally propagating the transformed cells either in a culture or by regenerating whole transgenic or chimeric plants; and (iv) recovering and optionally purifying the human hemoglobin protein that includes a complex consisting of the protein or proteins coded for by the nucleic acid and at least one iron-containing polphyritic nucleus, or a plurality of such complexes.

Method for producing human hemoglobin proteins using plant cells

Growing community of inventors

Volvic, France

Bertrand Merot