Avl Medical Instruments Ag

Roche Diagnostics Operations, Inc.

F. Hoffmann-la Roche Ag

Roche Diagnostics Gmbh

Bernhard Schaffar

Christoph Ritter

Wolfgang Huber

Andreas Martin Dolezal

Helmut Offenbacher

Hellfried Karpf

Herbert Kroneis

Werner E Ziegler

Heinz Kontschieder

Taghi Noormofidi

Anton Mostl

Gernot Florian

Marie-Luise Schinnerl

Klaus Reichenberger

Thomas Meier

Wolf-Dietrich Steinbock

Bernd Nidetzky

Marco Jean Pierre Leiner

Massoud Ghahramani

Marco Jean-Pierre Leiner

Wolf-Dietrich Steinböck

Johann Harer

Albert Romann

Thomas Stoisser

Gregor Steiner

Wolf-Dietrich Steinb&ouml;ck

Franz-Josef Krysl

G&uuml;nther Pucher

Marcus J Leiner

Robert Poettler

Stefan Leitgeb

M Jean-Pierre Leiner

Robert Pöttler

Franz Josef Krysl

Thomas Mag Rer Nat Stoisser

Methods and systems for hydrating and calibrating an electrochemical sensor are disclosed. The method comprises providing an electrochemical sensor, providing a calibration solution to a measuring chamber of the electrochemical sensor, contacting the sensor with the calibration solution within the measuring chamber, exposing the sensor and the calibration solution to an elevated temperature, and over a cooling period, cooling the sensor and the calibration solution with a tempering device, wherein the temperature is controllable within a device error range of temperature, performing a plurality of calibrations of the sensor, wherein a calibration of the plurality of calibrations is valid for a calibration margin of temperature error, the calibration margin of temperature error exceeds the device error range of temperature, and controlling a decrease of the temperature of the sensor and the calibration solution not to exceed the calibration margin of temperature error between consecutive calibrations of the sensor.

Method and system for hydrating and calibrating an electrochemical sensor

The present disclosure relates to a mutant lactate oxidase having increased stability, a nucleic acid encoding the mutant lactate oxidase, an expression vector comprising the nucleic acid, a host cell comprising the nucleic acid or the expression vector, a method of determining lactate in a sample, the use of the mutant lactate oxidase for determining lactate, a device for determining lactate in a sample using the mutant lactate oxidase and a kit for determining lactate comprising the mutant lactate oxidase.

Mutant lactate oxidase with increased stability and product, methods and uses involving the same

The invention concerns a sensor cassette that can be inserted into an analyzer comprising a continuous measuring channel for receiving fluidic media and sensory elements for determining chemical and/or physical parameters of the fluidic media. According to the invention the sensor cassette consists of at least two permanently connected but separately manufactured modules which each have a housing and a measuring channel section wherein the measuring channel sections of adjacent modules are connected to the continuous measuring channel by a fluidic coupling and wherein at least one of the connected modules is designed as a sensor module and has a sensor array comprising at least two sensory elements. Furthermore, a memory element is allocated to the sensor cassette on which specific information for the sensor cassette in particular with regard to its construction from the respective modules is stored.

Modular sensor cassette

In a method for calibrating a biosensor for the amperometric determination of creatinine in biological liquids, consisting of one electrode system each for measuring the concentrations of creatinine and creatine, with at least two calibration solutions being used, the electrode system for measuring the creatinine concentration is calibrated with a creatinine solution which, prior to the calibration measurement, was mixed in the form of an acidic solution with an alkaline buffer solution, and the electrode system for measuring the creatine concentration is calibrated with a solution in which creatinine and creatine are at a thermodynamic equilibrium.

Creatinine sensor calibration

An electrochemical sensor includes an electrochemically active sensor layer (sensor spot), which is applied by means of thick film techniques in at least one region of an electrically insulating, planar substrate, the surface of the sensor layer being brought into contact in a measuring area with the aqueous sample to be determined. At least one conductive path for signal pick-up is also applied on the substrate by means of thick film techniques. The sensor layer contains at least one oxide of metal from subgroups and wof the Periodic Table as sensor component. The contact between the electrochemically active sensor layer and the conductor is effected via an electrically conductive bridge layer which extends from the measuring area in a direction essentially parallel to the surface of the substrate. The bridge layer consists of corrosion-resistant material.

Electrochemical sensor

An electrochemical measuring device comprises an essentially planar sensor substrate with at least one electrochemical sensor, and a cover part in which a tunnel-shaped measuring channel is formed. At least one guiding groove is provided in parallel with the measuring channel. A sealing element which is positioned between the sensor substrate and the cover part to seal the measuring channel is provided with guiding bodies along its length, at least one of which guiding bodies projects into the guiding groove of the cover part. The sealing element is provided with narrow sealing lips bounding the measuring channel. Each short side of the sealing element has a guiding body projecting into recesses in the sensor substrate. The guiding bodies are disposed on that side of a sealing plane defined by the sealing lips, which faces away from the cover part.

Electrochemical measuring device with an planar sensor substrate

A device for performing electrochemical and/or optical measurements in fluids, includes a sensor part of substantially planar configuration carrying a plurality of sensors that are connected to conductive paths; a cover part, which includes a groove-shaped measuring channel through which the fluid will flow; and a sealing element positioned between the sensor part and the cover part for sealing the measuring channel. The cover part includes grooves which extend in parallel with the measuring channel and which define respective ridges between the grooves and the measuring channel, and the sealing element substantially includes guiding bodies which are retained in the grooves of the cover part and are provided with integrally molded narrow sealing lips protruding in the area of the ridge. The sensor part and the cover part are joined by a plurality of locking elements which are distributed along the measuring channel.

Device for performing measurements in fluids

Diagnostic flow through cell device

Biological components such as enzymes are immobilized in a three-dimensional cross-linked hydrophobic polymer. An enzyme is mixed with an aqueous solvent and a non-crosslinked prepolymer having an essentially nonpolar main chain with attached polar hydrophilic groups and cross-linking groups. The resultant mixture is exposed to cross-linking temperatures to react the prepolymer via the cross-linking groups without additional catalyst or cross-linking agents, and the aqueous solvent is evaporated to form a three-dimensional cross-linked hydrophobic polymer matrix containing the enzyme. The prepolymer can be an oil alkyl resin containing the main chain with attached polar hydrophilic groups and cross-linking groups. The oil alkyl resin is cross-linked by autoxidation, and aqueous solvent is evaporated to form the polymer matrix. A biosensor is prepared having the enzyme-containing polymer matrix on the surface of a supporting layer which contains cross-linking groups that improve adhesion between the supporting layer and the polymer matrix. Catalytic particles or charge conducting particles may be in the polymer matrix, and multiple polymer matrixes with different enzymes may be formed.

Preparation of a hydrophobic polymer matrix containing immobilized

A planar sensor for determining a chemical parameter of a sample, includes a substrate whose surface is at least partly plane and is provided with at least one potentiometric or amperometric and, possibly, an optical transducer, and one or more biochemical components. The transducer and the biochemical component are provided on the surface of the substrate, or at least part of the surface, as a sensor spot, and a cover membrane surrounding this sensor spot, is heat welded to the surface of the substrate. Those sensor spots that include a potentiometric or amperometric transducer are in contact with a strip conductor attached to the plane surface of the substrate and the thermal seal of the cover membrane is interrupted where the conducting strips lead away from the sensor spots.

Planar sensor for determining a chemical parameter of a sample

Method for analyzing gaseous or liquid samples, utilizing a one-way measuring element with a measuring channel containing at least one optical or electrochemical sensor and being provided with sealing elements on either end. In order to obtain accurate measurements in a simple manner the proposal is put forward that for measuring purposes a storage medium in the measuring channel be replaced by a separating medium which should then be replaced by the sample. Sample and storage medium will remain in the measuring element when it is discarded.

One-way measuring element for analyzing gaseous or liquid samples

Method for analysing gaseous or liquid samples, utilising a one-way measuring element with a measuring channel containing at least one optical or electrochemical sensor and being provided with sealing elements on either end. In order to obtain accurate measurements in a simple manner the proposal is put forward that for measuring purposes a storage medium in the measuring channel be replaced by a separating medium which should then be replaced by the sample. Sample and storage medium will remain in the measuring element when it is discarded.

Method for analyzing gaseous or liquid samples and a one-way measuring

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Graz, Austria

Bernhard Schaffar