Roche Diagnostics Operations, Inc.

Bernhard Rexer

Johann-Peter Thalhofer

Claus S Schneider

Frank Geipel

Stephan Glaser

Hellmut Eckstein

Stephanie Ronning

Rainer Muller

Michael Ratka

Claudia Giessel

Helmut Schoen

Guenter Guertler

A method for producing solid proteinase K which is insoluble in water and has high purity, the method comprising the steps of adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid, and isolating the solid proteinase K.

Highly purified proteinase K

The present invention concerns a method for producing recombinant trypsin from porcine pancreas inwhich is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to &#x3b2;-trypsin and autolysis of &#x3b2;-trypsin by &#x3b1;-trypsin into &#x3b5;-trypsin and from there into inactive peptides.

Method for producing recombinant trypsin

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Weilhem, Germany

Bernhard Rexer