Sandoz Ag

Boehringer Ingelheim Rcv Gmbh & Co. Kg

Bernhard Auer

Clemens Achmüller

Philipp Wechner

Alois A Jungbauer

Rainer Hahn

Waltraud Kaar

Rainer Schneider

Michael Seifert

Florian Werther

Silvio Podmirseg

Maria Reitmeir

Clemens Achmueller

Michael Sponring

Alois Jungbauer

Disclosed is a method for producing a recombinant protein of interest, the method being characterized in by the following steps:

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Disclosed is an affinity matrix comprising a solid phase and an affinity ligand comprising peptide bonds coupled to this solid phase, wherein the affinity ligand comprising peptide bond is selected from the following group of ligands: a) peptides comprising the formula XXXX, wherein Xto Xare amino acid residues and at least two of Xto Xis W, Y or F; b) peptides comprising the formula XXXX, wherein Xto Xare amino acid residues, at least one of Xto Xis W, and at least one of Xto Xis E or D; and c) poly-amino acids consisting of an amino acid monomer of the group consisting of R, K, E and D and an amino acid monomer of the group consisting of Y, F and W, preferably poly-KY, poly-KF, poly-KW, poly-RY, poly-RF, poly-RW, poly-EY, poly-DY, poly-EF, poly-EW, poly-DF and poly-DW, with the proviso that the peptides according to a) and b) have a maximum length of 35 amino acid residues and that the poly-amino acids according to c) have a minimum length of 20 amino acid residues.

Affinity ligands

The invention relates to a process for the recombinant production of a heterologous polypeptide of interest by cultivating a bacterial host cell transformed with an expression vector comprising a nucleic acid molecule encoding a fusion polypeptide wherein (a) the amino-proximal fusion partner is an autoprotease Ncomprising the replacement(s) by glutamic acid of one or more cysteines at positions corresponding to the positions 112, 134, and 138 of the autoprotease Nof classical swine fever virus and (b) the carboxyl-proximal fusion partner is an heterologous polypeptide of interest fused to the autoprotease Nso that it is capable of being cleaved from the fusion polypeptide by autoprotease Nautoproteolytic activity, said process comprising (i) cultivating the transformed host cell under conditions permitting the expression of the fusion polypeptide and the formation of corresponding cytoplasmic inclusion bodies, (ii) isolating the inclusion bodies from the host cell, (iii) solubilizing the isolated inclusion bodies, (iv) inducing autoproteolytic cleavage of the heterologous polypeptide of interest from the fusion polypeptide, and (v) isolating the cleaved heterologous polypeptide of interest.

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Innsbruck, Austria

Bernhard Auer