Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

Bernhard Angerer

Waltraud Ankenbauer

Frank Laue

Gudrun Schmitz-Agheguian

Christine Ebenbichler

Vitaly Svetlichny

Elizaveta Bonch-Osmolovskaya

Astrid Reiser

Ursula Markau

A purified thermostable enzyme is derived form the thermophilic archaebacterium. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95&#xb0; C. in the presence of stabilizing agents and possesses 3'-5&#x2032; proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).

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A purified thermostable enzyme is derived from the thermophilic archaebacteriumThe enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95&#xb0; C. in the presence of stabilizing agents and possesses 3'-5&#x2032; proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).

A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3&prime;-5&prime; exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.

Thermostable DNA polymerase from carboxydothermus hydrogenoformans

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Rosenheim, Germany

Bernhard Angerer