Boehringer Mannheim Gmbh

Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Bernd Vogt

Joachim Ziegenhorn

Joachim Siedel

Lieselotte Schellong

Rupert Herrmann

Christian Klein

Herbert Von Der Eltz

Hans-Georg Batz

Uwe Kobold

Peter Sluka

Martina Junius-Comer

Thomas Duelffer

Rudolf Portenhauser

Liselotte Schellong

Wolfgang Treiber

Hans-Dieter Lessmann

The present invention relates to a reagent for digestion of hemoglobin comprising a buffer, pepsin and a-dialkyl-imidazolium salt. It also discloses to the use of this reagent in a method for digesting hemoglobin, in a method for detecting HbA1c, and to a sampling tube for collection of a whole blood sample comprising said reagent for digesting hemoglobin.

Reagent for digestion of hemoglobin

Novel compounds having the general formula

Alkyl-substituted aryl-saccharides, surface-active agents which contain

The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto. The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.

Reagent for specific determination of fructosamine

The present invention provides a colorimetric process for the detection of an oxidative coupling reaction in which a coupling component is reacted with a developer component in the presence of an oxidation agent to give a colored material, wherein, as coupling component, there is used a compound of the general formula: ##STR1## in which X is a bromine or chlorine atom, n is 1, 2 or 3, Y is COOR, CONRR', SO.sub.3 R, SO.sub.2 NRR', OR, NRR' or NRR'R'.sym. and R, R' and R', independently of one another, are hydrogen atoms or C.sub.1 -C.sub.3 -alkyl radicals. The present invention also provides a reagent which contains the above compound, as well as new substituted phenols and a process for the preparation thereof.

Substituted phenols

This invention teaches a process for reducing protein matrix effects in assays for serum fructosamine. Blood or blood derived samples are used, and one adds two reagents, one of which reduces interference caused by non-specific reducing substances, the other of which eliminates turbidity. Incubation follows, and then the pH of the sample is adjusted and color forming reagent is added. In one embodiment, the incubation time is only 1-15 minutes. In another embodiment, the first reagent contains peroxidase.

Process for reduction of the matrix effect in a fructosamine

Process for the specific determination of the serum fructosamine content in blood or in samples obtained from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, in which before the color reaction sample components with a non-specific reducing action and/or causing turbidity are removed and subsequently the color reagent is added at a pH value of from 10 to 12. The sample components are removed by treatment at approximately neutral pH value with a reagent composition comprising at least one enzymatic oxidation agent, optionally together with peroxidase and/or catalase and/or lipase, as well as with at least one SH group-blocking substance. A kit for the specific determination of the serum fructosamine content in blood or samples obtained from blood, comprises said reagent composition, a rebuffering reagent with a buffer which has an alkaline pH value and a color reagent for the detection of fructosamine.

Process and reagent for the specific determination of fructosamine

The present invention provides a process for the determination of fructosamine in body fluids using as a standard, a solution which contains a peptide or protein, the amino acid units of which consist of at least 25% of lysine and/or ornithine and is present in glycosylated form. The present invention also provides a standard solution for the determination of fructosamine containing a peptide or protein, the amino acid unit content of which consists of at least 25% lysine and/or ornithine and is present in glycosylated form, and a method of calibrating such a standard solution.

Method for determining the concentration of fructosamine using a

For the determination of fructosamine in body fluids a solution containing fructosamine and albumin is used as the standard solution for calibration which standard solution is essentially free of glucose and which has a pH between 5.0 and 6.0 and contains at least 10 mmol/l buffer.

Process for the determination of fructosamine in body fluids and

A process for the determination of fructosamine in body fluids by the reaction of a sample solution with a color reagent, wherein the sample liquid is mixed with a buffer solution having a pH value of from 9 to 12, a color-forming reagent and uricase, as well as with at least one detergent, and the chronological change of the extinction is measured kinetically in a temperature range of from 20.degree. to 40.degree. C. at the earliest after 5 minutes.

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Tutzing, Germany

Bernd Vogt