Aventis Research & Technologies Gmbh & Co Kg

Hoechst Aktiengesellschaft

Aventis Pharma Deutschland, Gmbh

Bernd Kirschbaum

Wilfried Schwab

Robert R Bartlett

Stefan Muellner

Stefan Mullner

Irvin Winkler

Wilhelm Stahl

Stefan M&uuml;llner

Michael Meisterernst

The present invention relates to the identification and molecular-biological and biochemical characterization of novel DEAH-box proteins which show structural similarity with other known helicases. The invention also relates to DNA sequences encoding such proteins. It is demonstrated that such proteins bind ATP and nucleic acid and possess helicase and ATPase activities. This invention further relates to processes for preparation of such proteins and their use in pharmacologically relevant test systems and therapeutic applications.

DEAH-box proteins

Isoxazole and crotonamide derivatives and their use as pharmaceuticals and diagnostics

An process for analyzing transcription which can be automated and which is suitable for bulk screening. The process involves transcribing a DNA sequence using a nuclear extract, which can be complemented or fully replaced by exogenous transcription factors and/or cofactors; optionally removing the proteins of the reaction mixture; binding the resulting transcript to a solid matrix; removing the excess labeled nucleotides; and determining the amount of labeled transcript. Methods of using the inventive process to identify compounds having a selective effect on gene expression.

In-vitro transcription processes for screening natural products and other chemical substances

A compound of the formula ##STR1## where R.sup.1 is the radical of the formula II or III ##STR2## is suitable for the production of a pharmaceutical for the treatment of inflammations, carcinomatous diseases, or autoimmune diseases. A compound of the formula IV ##STR3## is suitable for the production of specific antibodies against a compound of the formula I for the discovery of specific-binding proteins from cell extracts, serum, blood, or synovial fluids, for the purification of proteins, for the modification of microtiter plates, or for the preparation of chromatography material, in particular of affinity chromatography material, and for use in diagnostics.

Isoxazole and crotonamide derivatives and their use as pharmaceuticals

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Mainz, Germany

Bernd Kirschbaum