Roche Sequencing Solutions, Inc.

Max-delbruck-centrum Fur Molekulare Medizin

Max-delbrueck-centrum Fuer Molekulare Medizin

Metagen Pharmaceuticals Gmbh

Signature Diagnostics Gmbh

Signature Diagnostics Ag

Bernd Hinzmann

Horst Will

Sophie Beckert

John Lee

John Palma

Bronwen Miller

Alexander Lovejoy

Armin Schmitt

Andr&eacute; Rosenthal

Hans-Peter Adams

Victoria Constandse

Melissa Loyzer

Daniel Klass

Urich-Peter Rohr

Andre Rosenthal

Aarthi Balasubramanyam

Edgar Dahl

Christian Pilarsky

Thomas Specht

Djoerk-Arne Clevert

André Rosenthal

Djörk-Arné Clevert

Present disclosure provides a method including isolating DNA from a source, thereby providing a composition including the isolated DNA. The isolated DNA has at least first and second target regions, where the length of the second target region is greater than the length of the first target region. The method further includes quantifying a total mass of the isolated DNA, quantifying a first quantification cycle (C) of the first target region and a second Cof the second target region, and calculating a Q-ratio for the isolated DNA by dividing the second Cby the first C. The method further includes determining a value for a quality-mass constant (k), estimating a required input mass by dividing kby the Q-ratio, and preparing the isolated DNA for sequencing if the total mass of the isolated DNA in the composition is equal or greater than the required input mass.

System and method for isolation and qualification of nucleic acids

The invention is a method of predicting recurrence of colorectal cancer in a patient following surgery, the method comprising analysis of circulating tumor DNA from a patient's sample.

Non-invasive test to predict recurrence of colorectal cancer

The present invention provides method essentially comprising the steps of (i) providing a DNA sample, (ii) providing at least a first pair of amplification primers which is capable of generating a first amplicon from a LINE sequence that has a size of less than 80 bp, (iii) providing at least a second pair of amplification primers which is capable of generating a second amplicon from a LINE sequence that has a size of more than 160 bp, (iv) performing a qPCR and determining cq values for each of the generated amplicons, and (v) determining the relative concentrations of said amplicons.

Quality testing of DNA samples

Human nucleic acid sequences&mdash;mRNA, cDNA, genomic sequences&mdash;from endometrial tumor tissue, which code for gene products or portions thereof, and their use, are described. In addition, the polypeptides that can be obtained by way of the sequences and their use are described.

Human nucleic acid sequences from endometrial tumor tissue

DNA sequences for human matrix metalloproteases are disclosed, as well as homologous DNA sequences homologous and derived therefrom. Also disclosed are the proteins and protein variants coded by these DNA sequences, there expression, preparation and use. The invention has applications in the fields of biomolecular, medical and pharmaceutical research, for medical diagnosis and therapy, and in the pharmaceutical and biotechnological industry.

DNA sequences for matrix metalloproteases, their production and use

Growing community of inventors

Berlin, Germany

Bernd Hinzmann