Dyomics Gmbh

Pierce Biotechnology, Inc.

Carl Zeiss Jena Gmbh

Bernd G Schweder

Peter T Czerney

Matthias S Wenzel

Frank G Lehmann

Wilhelm G Frank

Surbhi Desai

Brian David Wolf

Boguslawa Dworecki

Zakir S Murtaza

The invention concerns compounds based on benzoxazoles wherein selected substituents are each a reactive group A bound via a linker L and adapted to covalently bond a compound of the invention to a to-be-marked molecule K, wherein A is an amine, hydroxy or phosphoramidite function, a carboxylic acid, an alkyl or active ester derived therefrom; a carboxylic acid hydrazide; or a carboxylic acid amide where K is a covalently bound component selected from the group haptenes, proteins (antibodies), low molecular weight drug compounds, peptides, nucleotides, nucleosides, DNA oligomers, polymers, and t is from 1 to 10, and L is a linker selected from a group featuring &#x2014;(CH)&#x2014;, &#x2014;[(CH)&#x2014;O]&#x2014;(CH)&#x2014;, &#x2014;NR10-(CH)&#x2014;, &#x2014;O&#x2014;(CH)&#x2014;, &#x2014;S&#x2014;(CH)&#x2014;, &#x2014;NR10-C(O)&#x2014;(CH)&#x2014;, &#x2014;NR10-C(O)&#x2014;O&#x2014;(CH)&#x2014;, &#x2014;NR10-C(O)&#x2014;NR11-(CH)&#x2014; or &#x2014;SO&#x2014;NR10-(CH)&#x2014;, wherein R10 and R11 are each independently hydrogen, alkyl and alkoxy-alkyl (&#x2014;[(CH)&#x2014;O]&#x2014;CH), &#x3c9;-sulfoalkyl (&#x2014;(CH)&#x2014;SO), m represents the numbers of 2-5, p, r and s each independently represent the number of 1-10.

Marker dyes for UV and short wave excitation with high stokes shift based on benzoxazoles

Compounds used as dyes comparable to Alexa Fluor 350 dyes. The inventive compounds have high fluorescence quantum yield and high photostability. The dyes facilitate analysis of biological structures with enhanced sensitivity and selectivity.

Compounds used as dyes comperable to Alexa Fluor 350 dyes

A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.

Protein detection and quantitation using hydroxyquinolone dyes

Compounds, compositions, and methods for optical, including fluorescence optical, determinations useful in labelling biomolecules such as protein and deoxyribonucleic acid for their detection and quantitation. The compounds are diasteromeric cyanines with high hydrophilicity and other desirable properties.

Hydrophilic labels for biomolecules

The invention relates to fluorescent dyes (fluorophores) based on polymethines for use in optical measurement and detection procedures, in particular those employing fluorescence, for example in medicine, in pharmacology and in the biological, materials and environmental sciences. The objective was to create fluorophores based on polymethines that have a large Stokes shift, high photostability, long storage life and a high fluorescent quantum yield, and that can be excited in the simplest possible manner by white-light sources or laser radiation in the UV, visible or NIR spectral region. According to the invention dyes on the basis of polymethines having the general formulas I, II or III are employed.

Compounds based on polymethines

Coumarine/chinolone and Martina-type compounds, compositions, and method of using the compounds and compositions for protein detection, assay, quantitation, etc. are disclosed. The composition, also referred to as a dye, has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc.

Protein probe compounds, compositions, and methods

The present invention relates to the field of optical-fluorescent markers, particularly to benzopyrylo-polymethine-based optical-fluorescent markers.

Benzopyrylo-polymethine-based hydrophilic markers

The disclosure is directed to so-called laser-compatible NIR marker dyes based on polymethines for use in optical, in particular, fluorescence optical determination and detection methods, for example, in the fields of medicine, pharmaceutics and in the areas of life science, materials science and environmental science. The disclosure further discusses the aim of the invention which was to create NIR marker dyes based on polymethine which have a high degree of photostability and stability in storage as well as a high fluorescent yield and which can be excited to fluorescence in the easiest possible manner by means of laser radiation in the visible or NIR spectral range, particularly with light of an argon, helium/neon, or diode laser. Dyes based on polymethine of general formula (I) are used.

Growing community of inventors

Jena, Germany

Bernd G Schweder