Roche Diagnostics Operations, Inc.

Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-La Roche Ag

Bernd Buchberger

Manfred Watzele

Horst Donner

Claudia Kirr

Frank Bergmann

Horst Menzler

Hans Schels

Alois Rainer

Maximilian Ruhstorfer

Ulrike Fischer

Wolfgang Mutter

Albert Roeder

Nina Lassonczyk

Stephanie Wessner

Cordula Jany

The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. Preferred protein denaturing agents are detergents and chaotropic substances.

Inhibition of peroxidase enzymatic activity

The invention concerns a method for isolating and purifying nucleic acids from large sample volumes and a device suitable for this. The device comprises a funnel, a separating column, and a tab which are detachable from each other. The method according to the invention in which the device according to the invention is used ensures a higher yield of nucleic acids.

Method and device for purifying nucleic acids

The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. preferred protein denaturing agents are detergents and chaotropic substances.

The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

Use of acetals for isolation of nucleic acids

The present invention concerns a matrix reactor for reactions in which low-molecular and high-molecular reactants are involved wherein the reactor space Vcontains a matrix and comprises a reaction compartment and a supply compartment, wherein the matrix is composed of a porous material which can take up low-molecular reactants and wherein the exclusion volume Vof the matrix is available as the reaction compartment and the matrix volume Vof the matrix is available as the supply compartment. The matrix reactor can be used for enzymatic processes, in particular for a coupled in vitro transcription/translation reaction to produce proteins.

Matrix reactor and a method for producing products in said reactor

The invention is a multichamber microdialysis device with a multitude of sample chambers in close side by side arrangement (similar to a microtiter plate) for receiving liquid samples, and at least one dialysate chamber for receiving a dialysate liquid. The sample chambers are surrounded by circumferential sidewalls and are in liquid exchange contact to a neighboring dialysis chamber via an exchange opening covered by a semipermeable membrane. The semipermeable membrane is fixed liquid-tight to the walls of the sample chamber, so that a diffusion exchange between the sample chamber and the correspondingly neighboring dialysate chamber is only possible via the membrane. Consequently, only molecules with a molecular weight below the molecular cut-off of the semipermeable membrane can diffuse from the sample chamber to the dialysate chamber or from the dialysate chamber to the sample chamber.

Multichamber microdialysis device

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Peissenberg, Germany

Bernd Buchberger