Keygene N.v.

Bernarda Gerharda Johanna Fierens-onstenk

Michiel Theodoor Jan De Both

Rik Hubertus Martinus Op Den Camp

Bjorn Alexander Kloosterman

Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.

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Wageningen, Netherlands

Bernarda Gerharda Johanna Fierens-onstenk