University of California

The United States Government as Represented by the Department of Veterans Affairs

Microbedx, Inc.

Bernard M Churchill

David A Haake

Yang Li

Joseph Chihping Liao

Edward F Wahl

Colin Halford

Jane T Babbitt

Marc A Suchard

Mitra Mastali

Daniel Gussin

Gabriel K Monti

Colin W Halford

Described are probes and methods for detecting antibiotic susceptibility of a specimen. The method comprises contacting the specimen with an oligonucleotide probe that specifically hybridizes with a target nucleic acid sequence region of ribosomal RNA. The target sequence is at the junction between a pre-ribosomal RNA tail and mature ribosomal RNA of 23S or 16S rRNA. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic susceptibility.

Antibiotic susceptibility testing using probes for preribosomal RNA

Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.

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A system for patient diagnostic testing includes the physiological data output is in a two dimensional graphical format. Optionally, the report includes imaging data. Color is a dimension provided to the graphical presentation of normal patient data in horizontal and vertical dimensions. Detrusor pressures are obtained. One detrusor pressure is the difference between average data points of the bladder and rectal pressures. The other is obtained by subtracting curve fitted rectal and bladder pressures. The displayed data is a presentation including bladder capacity information, pressure characteristics, and data as a function of volume such that bladder volume is indicated.

Video data representation of physiological data

Growing community of inventors

Pacific Palisades, CA, United States of America

Bernard M Churchill