Biomerieux, Inc.

Biomerieux Sa

Bernard Jean-Marie Limon

Christine Rozand

Marie-Pierre Montet

Jean-Pierre Flandrois

Bruno Colin

Charles H Rogers

Patrick Broyer

Cécile Paris

Philippe Renaud

Fabienne Marquis-Weible

Method for isolating microorganism from a sample likely contaminated by microorganism, including: (a) device for isolating microorganisms including a bottom waterproof layer, a nutritional layer, which is placed on the bottom layer and includes a dehydrated culture medium, an isolation layer which is pervious to elements included in the nutritional layer and is capable of retaining the bacteria on the surface and covering all or part of the nutritional layer, and a top protective layer; (b) depositing a volume of the sample on the isolation layer; (c) isolating the microorganisms by impoverishing or layering the sample using an isolating device; (d) incubating the device for an amount of time at a temperature to enable growth of microorganisms, method including at least one step of rehydrating the culture medium using a volume of liquid before or with step b) and/or c) and/or d), before or simultaneously with step b) and/or c).

Method for isolating microorganisms on a culture medium, and related device

A reaction card () having a body, a front surface () and a rear surface () defined by an edge (), at least one inlet (and/or) and at least one outlet (and/or), connected by a network of channels () constituting at least one reaction path for at least one fluid which is directed via valves (toand/or); each valve includes a flexible film () which can be deformed to allow fluid passage, the film being fixed on the card's rear surface at a peripheral indentation of an assembly of channels associated with the valve; the card includes channels flush with at least one of its surfaces, the channels being of two different cross-sections, a small cross-section serving as a reaction compartment; each front or rear surface is delimited by at least one film (and/or).

Reaction card and use of same

Analysis card

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Rignat, France

Bernard Jean-Marie Limon