Illumina Corporation

Axys Pharmaceuticals, Inc.

Merck Sharp + Dohme Corp.

Merck Frosst Canada, Inc.

Merck Canada Inc.

Bernard Hirschbein

Wenyi Feng

Poorya Sabounchi

Helmy A Eltoukhy

Tarun Kumar Khurana

Robert C Kain

Randall Smith

Mark R Pratt

Joseph Francis Pinto

Tracy Helen Fung

Xiaohai Liu

Jonathan Mark Boutell

Geoffrey Paul Smith

Xiaolin Wu

Min-Jui Richard Shen

Kay Klausing

Thomas J Joseph

Carolyn Tregidgo

Michael J Green

Cheuk K Lau

Michel Therien

Jacques Yves Gauthier

Serge Leger

Cameron Black

Chun Sing Li

James T Palmer

Christophe Mellon

Chitra Baskaran

Dan McKay

Vouy-Linh Truong

James William Janc

Christopher I Bayly

Filiz Gorpe-Yasar

Tracy H Fung

Tarun Khurana

Thomas Joseph

Joseph Pinto

Yir-Shyuan Wu

Xi-Jun Chen

Vouy Linh Truong

Yasaman Farshchi

Dan Mckay

James W Janc

An example of a flow cell includes a substrate and a cationic polymeric hydrogel on the substrate. The cationic polymeric hydrogel includes a cationic moiety that is i) integrated into a monomeric unit of an initial polymeric hydrogel or ii) attached to the monomeric unit of the initial polymeric hydrogel through a linker. The flow cell further includes an amplification primer attached to the cationic polymeric hydrogel.

Flow cells

A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

Biosensors for biological or chemical analysis and systems and methods for same

There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

Detector with reduced fluorescence range noise

The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Methods and compositions for nucleic acid sequencing

A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.

Method of analyzing signal data from a biosensor including a detection device having an array of light detectors. The method includes obtaining signal data from the light detectors. The signal data includes light scores that are based on an amount of light detected by the light detectors during a plurality of imaging events. The method also includes analyzing the light scores from a group of light detectors for each of the plurality of the imaging events. The method also includes determining respective crosstalk functions of the light detectors in the group. Each of the crosstalk functions for a corresponding light detector is based on the amount of light detected by other light detectors in the group. The method also includes analyzing the signal data for each of the imaging events using the crosstalk functions to determine characteristics of the analytes-of-interest.

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San Francisco, CA, United States of America

Bernard Hirschbein