Biomerieux, Inc.

Centre National De La Recherche Scientifique

Biomerieux Sa

Elicityl

Université Paris—sud

Instituto Superior Tecnico

Research Foundation for Medical Devices

Bernard F Mandrand

Herve Perron

Frederic Bedin

Frederic Beseme

Francois Mallet

Thierry Delair

Abdelhamid Elaissari

Glaucia Paranhos-Baccala

Christian Pichot

Florence Komurian-Pradel

Marie-Helene Charles

Colette Jolivet-Reynaud

Philippe Cros

Guy Oriol

Jérôme Bibette

Olivier Mondain-Monval

Marie-Noelle Erout

Eric Bosc

Ahmed Arkis

Doran Spencer

Patrice A Allibert

Bruno Colin

Claude Mabilat

Carole Chaix

Alain Theretz

Francis Garnier

William McAllister

Pierre Imbaud

Silvère Bonnet

Stéphane Havet

Francedillaois Mallet

Patrick Broyer

Philippe L Cleuziat

Michel C Jolivet

Bernard Francois Mach

Alain Laurent

François Mallet

Hervé Perron

Pascal T Dalbon

Frédéric Bedin

Monique Jaubert

Francedillaois Rieger

Tomas Dobransky

Nicolas Spinelli

Ludovic Bastide

Mialy Randriantsoa

Mireille Sodoyer

Catherine Ott

Mylene Lesenechal

Corinne Moustrou

Benoît Darblade

Fran&ccedil;ois Mallet

Jean-Marie Tiercy

Philip William Tuke

Hafsa Korri-Youssoufi

Daniela Zsoldos

Marie-Thérèse Charreyre

José Manuel Gaspar Martinho

Jeremy Alexander Garson

Carine Malcus-Vocanson

Martine Perree-Fauvet

Jean-Pierre Mahy

Florence Sauzedde

Frédéric Canonne

José Paulo Sequeira Farinha

Paula Relogio

Jean-Philippe Zeris

Agn&eacute;s Perrin

Herv&eacute; Perron

Agnès Perrin

Nad&eacute;ge Arnaud-Barbe

Val&eacute;rie Cheynet-Sauvion

Aurelie Bouchet

Valérie Cheynet-Sauvion

Nadège Arnaud-Barbe

Franck Montagne

Colette Jolivet Reynaud

Hugues Brisset

Aude-Emmanuelle Navarro

Carole Chaix-Bauvais

Frédéric Beseme

The present invention provides a substrate for purifying a biological liquid comprising a solid phase onto which molecules of at least one oligosaccharide that is capable of binding to one or more antibodies are grafted by covalent bonding, said solid phase carrying free aldehyde functions &#x2014;CHO, a method of producing such a substrate, the use of such a substrate to stabilize the antibody/oligosaccharide bond, as well as a method of purifying a biological liquid in which the biological liquid is brought into contact with at least one substrate of the invention, in a manner such as to obtain the capture of antibodies present in said biological liquid by binding with at least certain of the molecules of oligosaccharide(s) grafted onto the solid phase.

Substrate for the purification of biological liquids

The present invention concerns a method of purifying a biological liquid comprising antibodies corresponding to whole human or animal blood or to a product obtained from human or animal blood, by bringing a biological liquid into contact with at least one substrate comprising a solid phase onto which molecules of at least one oligosaccharide are grafted by covalent bonding, the molecules of the at least one oligosaccharide being capable of binding to one or more of said antibodies present in said biological liquid, in a manner such as to capture at least a portion of said antibodies present in said biological liquid by binding with at least certain of the molecules of oligosaccharide(s) grafted onto said solid phase, characterized in that said solid phase carries free aldehyde functions &#x2014;CHO.

Method for the purification of whole blood or a blood-derived product

The invention relates to fluorescent polymers soluble in an aqueous solution carrying at least 5 fluorophores which are distributed on the polymer and which exhibit the following properties:

Fluorescent polymers soluble in an aqueous solution and a method for the production thereof

The invention relates to novel electropolymerisable monomers which are to be polymerised in an aqueous solution and comprise: an electropolymerisable pattern selected from acetylene, pyrrols, thiophenes, indols, anilines, azines, p-phenylene vinylenes, p-phenylenes, pyrenes, furanes, selenophenes, pyrridazines, carbazoles, acrylates, methacrylates and the derivatives thereof, and a metalloporphyrine which is substituted by at least two ionised or ionizable entities in an aqueous solution. The invention also relates to a method for the polymerization of such monomers, to the electroactive probe that can be obtained by the polymerization of such monomers, and to a method for detecting a target ligand in a biological sample using one such electroactive probe.

Electropolymerisable monomers, soluble in an aqueous solution and comprising a metalloporphyrin

The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z', which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, &#x3b1;-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell

An isolated polynucleotide having a nucleotide sequence selected from the group consisting of (a) SEQ ID NO: 21, (b) the full-length sequences encoding a polypeptide having a peptide sequence selected from the group consisting of SEQ ID NOs: 25 and 26, and (c) the full-length complementary sequences to the sequences set forth in (a) or (b).

Isolated multiple sclerosis (MS)-associated retrovirus (MSRV) nucleic acids corresponding to the gag region

Viral material, in the isolated or purified state, in which the genome comprises a nucleotide sequence chosen from the group including sequences SEQ ID NO:46, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53 and SEQ ID NO:56, their complementary sequences and their equivalent sequences, in particular nucleotide sequences displaying, for any succession of 100 contiguous monomers, at least 50% and preferably at least 70% homology with the said sequences SEQ ID NO:46, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53 and SEQ ID NO:56, respectively, and their complementary sequences.

Viral material and nucleotide fragments associated with multiple sclerosis, for diagnostic, prophylactic and therapeutic purposes

The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.

Method and device for detecting biomolecules

The invention relates to an electropolymerizable monomer, intended to be polymerized in aqueous solution, comprising a single electropolymerizable unit and an electron-donating group, characterized in that it also comprises at least one arm ionizable in aqueous solution. The invention also relates to the polymerization process, to the electroactive probe thus obtained and to the method for the detection of a target ligand in a biological sample.

Electropolymerisable monomers that are soluble in aqueous solution and electroactive probes that can be obtained with such monomers

The invention concerns a nucleic material, in isolated or purified state, and a nucleotide fragment comprising a nucleotide sequence selected from the group consisting in (i) the sequences SEQ ID NO: 112, SEQ ID NO:114, SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO. 124, SEQ ID NO: 130, SEQ ID NO: 141 and SEQ ID NO: 142, (ii) the complementary sequences of sequences (i); and (iii) the sequences equivalent to sequences (ii) and (iii), in particular the sequence having for every series of 100 contiguous monomers, at least 50%, preferably 70% homology with sequences (i) and (ii) respectively. The invention also concerns their uses for detecting a retrovirus associated with multiple sclerosis and/or rheumatoid arthritis.

Multiple sclerosis-associated retroviral (MSRV) nucleic acids corresponding to the Env region

Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses

The invention provides viral material and nucleotide fragments associated with multiple sclerosis and/or rheumatoid arthritis for use in method of diagnosis, prophylaxis, and therapy.

RNA may be transcribed using a nucleotide reagent as the promoter. The reagent may enable RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of polynucleotide matrix with a higher yield when the matrix is RNA than when the matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with the ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.

RNA-dependent RNA polymerase functioning preferably on a RNA matrix and promoter-dependent transcription process with said RNA-dependent RNA polymerase

The invention relates to novel pyrrole derivatives of the formula (I) which make it possible to immobilize and address oligonucleotides by electropolymerization. Said invention also relates to thus produced electroactive polymers and to methods for using them for detecting, identifying and dosing analytes in a sample. (I) wherein Ris one type of oligonucleotide, Y is S or O, X is a spacer arm selected from &#x2014;(CH)&#x2014;O&#x2014;, &#x2014;(CH)&#x3c;SB&#x3e;P&#x3c;/SB&#x3e;O&#x2014;[(CH)&#x2014;O]&#x2014;, &#x2014;(CH2); &#x2014;CO&#x2014;NR'&#x2014;(CH)&#x2014;O&#x2014;, &#x2014;CH&#x2014;NCH&#x2014;(CH)&#x2014;O&#x2014;, &#x2014;CH)CO&#x2014;NR&#x2032;&#x2014;[CH)&#x2014;O]&#x2014;, &#x2014;(CH)NCH[(CH)&#x2014;O]&#x3c;SB&#x3e;S&#x3c;/SB&#x3e;&#x2014;, R&#x2032; is H or CH, n is an integer number ranging from 1 to 5, p is an integer number ranging from 1 to 2, q is an integer number ranging from 1 to 4, r is an integer number ranging from 1 to 3, r&#x2032; is an integer number ranging from 1 to 3, s is an integer number ranging from 1 to 3, n, p, q, r, r&#x2032; and s are identical or different, a pyrrole cycle is substituted in a position 2, 3, 4 or 5.

Pyrroles substituted by oligonucleotides

The present invention relates to a method for simultaneously detecting hybridization reactions and immunoreactions in a sample which may contain target analytes consisting of at least one nucleic acid and of at least one other ligand that is different in nature, characterized in that it comprises the steps consisting in: The invention also relates to the use of this method in diagnosis and the biological or diagnostic test kits that are useful for carrying out the method of the invention.

Method for simultaneously detecting hybridization reactions and immuno-reactions, and its uses in diagnosis

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Villeurbanne, France

Bernard F Mandrand