Cytonome/st, LLC

Waters Technologies Corporation

Cytonome, Inc.

Markem-imaje Corporation

Bernard Bunner

John R Gilbert

Manish Deshpande

Geoff C Gerhardt

Andrew Johnson

Hugh Lewis

Sebastian Böhm

Theodore A Dourdeville

Martin Gilar

Joseph D Michienzi

Keith Fadgen

Charles T Murphy

Robert A Jencks

Joseph A Jarrell

Frances H Benton

Arjun Venkataramanan

Alan G Gibson

Richard Day

Keith Edward Fadgen

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

Multilayer hydrodynamic sheath flow structure

Industrial printing systems, including systems and techniques relating to drop-on-demand (DOD) inkjet printing systems include an apparatus including: a receptacle defining a hold chamber to receive ink, the receptacle including a first portion configured to hold a first quantity of ink, and a second portion that is smaller than the first portion and configured to hold a second quantity of ink, the second portion including a first heat conducting surface, and a second heat conducting surface offset from the first heat conducting surface by a distance determined in accordance with a melting point of the ink, the second heat conducting surface defining a barrier between the first portion and the second portion and including at least one opening configured to allow flow of the ink from the first portion to the second portion; and at least one heating element configured to heat the receptacle.

Systems and techniques for melting hot melt ink in industrial printing systems

An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.

Actuation of parallel microfluidic arrays

A method may include reducing fluid flow between a rotor and a microfluidic device. The method may further include reducing a sealing force between the rotor and the microfluidic device. The method may also include rotating the rotor relative to the microfluidic device, at the reduced sealing force, to change a fluid pathway therebetween. The method may additionally include reestablishing the sealing force to produce a fluid tight seal between the rotor and the microfluidic device. Moreover, the method may include reestablishing the fluid flow between the rotor and the microfluidic device.

Managing fluidic connections to microfluidic devices

A system that incorporates teachings of the subject disclosure may include, for example, a process that includes obtaining a porous medium comprising a porous material having a first shape and an initial porosity profile. The porous medium is engaged with a cavity in a fluidic device, wherein the cavity is in fluid communication with a channel of the fluidic device. The first shape of the porous material can be adjusted to a second shape resulting in the initial porosity profile being adjusted to a target porosity profile. Such adjustment can be accomplished by the engaging of the porous medium with the cavity, by pre-adjusting a shape of the porous media before insertion into the cavity, or by some combination thereof. Other embodiments are disclosed.

Device and methods using porous media in fluidic devices

Flow through pressure sensors for use in fluid chromatography systems include a planar device formed from diffusion bonding of a plurality of metallic sheets and at least one sensing element. The planar device has a top surface, a bottom surface and a flow through channel. A diaphragm formed from a portion of one of the top or bottom surfaces is located adjacent to a sensing region of the flow through channel and is attached to the sensing element. The diaphragm is sized to deflect a distance in response to fluid pressure in the sensing region, which has an internal volume of less than about 25 microliters. The diaphragm and attached sensing element form a pressure sensor that measures strain or deflection of the diaphragm to calculate a pressure within the sensing region.

Pressure sensing and flow control in diffusion-bonded planar devices for fluid chromatography

Provided are systems and methods for adapting to volume variations in microfluidic chromatography columns. A column is calibrated by comparing a parameter of the column with a same parameter of a reference column and generating, by a processor, an adjustment factor in response to the comparison between the parameter of the column with a same parameter of the reference column. Volume differences between the calibrated column and the reference column are compensated for by integrating the generated adjustment factor into a sample separation involving the calibrated column.

Systems and methods of compensation for chromatography column volume variations

Described are techniques for use in connection with analyzing a droplet. One or more droplets of a sample are formed on a surface of a digital microfluidic device. The droplets are manipulated to perform processing using said one or more droplets generating one or more resulting droplets. The one or more resulting droplets may be transferred from the microfluidic device to another device for analysis. The one or more droplets may also be provided to the digital microfluidic device from yet another device or analysis instrument.

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Watertown, MA, United States of America

Bernard Bunner